Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5607_RS13000 Genome accession   NZ_AP019782
Coordinates   2972271..2973410 (-) Length   379 a.a.
NCBI ID   WP_221047269.1    Uniprot ID   A0A8D5ANC1
Organism   Methylogaea oryzae strain E10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2967271..2978410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5607_RS12985 (MoryE10_25430) thrC 2967557..2968633 (-) 1077 WP_221047268.1 threonine synthase -
  K5607_RS12990 (MoryE10_25440) - 2968919..2969233 (+) 315 WP_054773773.1 late competence development ComFB family protein -
  K5607_RS12995 (MoryE10_25450) - 2969290..2972112 (-) 2823 WP_246598862.1 EAL domain-containing protein -
  K5607_RS13000 (MoryE10_25460) pilU 2972271..2973410 (-) 1140 WP_221047269.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5607_RS13005 (MoryE10_25470) sseA 2973494..2974354 (-) 861 WP_221047270.1 3-mercaptopyruvate sulfurtransferase -
  K5607_RS13010 (MoryE10_25480) - 2974539..2976956 (-) 2418 WP_221047271.1 calcium-binding protein -
  K5607_RS13015 (MoryE10_25490) - 2977048..2978175 (-) 1128 WP_221047272.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42306.36 Da        Isoelectric Point: 6.0685

>NTDB_id=73983 K5607_RS13000 WP_221047269.1 2972271..2973410(-) (pilU) [Methylogaea oryzae strain E10]
MEFKDYLRTLVTKDGSDIYIAVGAPPAAKFQGRLTPIDNVVLKPEDTKRMAYDLMSPEQQRDFEHKPEMNLAISEPGIGR
FRVNIFKQRNCVGMVIRNIKTDIPQADNLGLPPILKQVIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSGGHIITIED
PIEYVHPHKKSIVTQREVGVDTDTFEDALKNTLRQAPDVILIGEIRARETMEHAIAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLKAFVSQRLVPTVDGKRVAAIEILLGTPLVADTIRKGEVHMVKEVMEKSENLGMQTFDTAL
FNLYKEGRITLDEALRNSDSPNNLRLKINLSEETRDTARRESGLSDQAGSASLELIHDD

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=73983 K5607_RS13000 WP_221047269.1 2972271..2973410(-) (pilU) [Methylogaea oryzae strain E10]
ATGGAATTCAAAGATTACCTGCGGACCCTGGTGACCAAGGACGGTTCCGACATTTACATCGCCGTGGGTGCGCCGCCGGC
GGCCAAGTTCCAGGGCCGGCTGACCCCCATCGACAACGTGGTGCTCAAGCCGGAAGACACCAAGCGCATGGCTTACGACT
TGATGTCGCCGGAGCAGCAGCGCGACTTCGAGCACAAGCCGGAAATGAACCTGGCCATCAGCGAGCCGGGCATCGGCCGC
TTCCGCGTCAACATCTTCAAGCAGCGCAATTGCGTCGGCATGGTGATCCGCAACATCAAGACCGACATCCCGCAGGCGGA
CAACCTGGGCCTGCCGCCGATCCTGAAGCAGGTCATCATGGAGAAGCGCGGCCTGATCCTGTTCGTGGGCGGCACCGGCT
CCGGCAAGTCCACCTCCCTGGCGGCCCTGATCGACTACCGCAACAGCAATTCCGGCGGCCACATCATCACCATCGAGGAC
CCCATCGAATACGTCCATCCGCACAAGAAGTCCATCGTCACCCAGCGCGAGGTGGGGGTGGACACGGACACTTTCGAGGA
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAAATCCGCGCCCGCGAAACCATGGAACACG
CCATCGCCTTCGCCGAAACCGGCCACTTGTGCCTGTCCACCCTGCACGCCAACAACGCCAACCAGGCCCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGCAACCAGCTGTTGCTGGATTTGTCCTTGAACCTCAAGGCTTTCGTGTCGCAGCG
CCTGGTGCCCACGGTGGACGGCAAACGCGTGGCCGCCATCGAAATCCTGCTGGGCACGCCCTTGGTGGCGGACACCATCC
GCAAGGGCGAGGTGCACATGGTGAAGGAAGTAATGGAAAAGTCGGAAAACCTCGGCATGCAGACTTTCGACACGGCCCTG
TTCAACCTGTACAAGGAAGGCCGCATCACCCTGGACGAGGCCCTGCGCAACTCCGACTCGCCCAACAACCTGCGCTTGAA
AATCAACCTCAGCGAGGAAACCCGCGACACCGCCCGCCGGGAAAGCGGCCTCAGCGATCAAGCCGGCTCGGCCTCCCTGG
AGCTCATCCACGACGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.535

93.668

0.586
  pilU Acinetobacter baylyi ADP1

55.493

93.668

0.52
  pilU Vibrio cholerae strain A1552

51.576

92.084

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

91.029

0.401

Multiple sequence alignment