Detailed information NTG0739744

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH532_RS22365
• Genome accession: NZ_CP107379
• Coordinates: 4493614..4494414 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_30
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4488614..4499414

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH532_RS22330	gspH	4488770..4489285 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH532_RS22335	gspI	4489282..4489647 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH532_RS22340	gspJ	4489644..4490240 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH532_RS22345	gspK	4490237..4491223 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH532_RS22350	gspL	4491210..4492403 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH532_RS22355	-	4492396..4492881 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH532_RS22360	-	4492871..4493617 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH532_RS22365	pilD	4493614..4494414 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH532_RS22370	hrpB	4494462..4496891 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH532_RS22375	thpR	4496964..4497500 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH532_RS22380	sfsA	4497500..4498216 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH532_RS22385	dksA	4498379..4498834 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=739744 OH532_RS22365 WP_002888947.1 4493614..4494414(+) (pilD) [Klebsiella pneumoniae strain CRKP_30]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=739744 OH532_RS22365 WP_002888947.1 4493614..4494414(+) (pilD) [Klebsiella pneumoniae strain CRKP_30]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425