Detailed information NTG0737403

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MTS96_RS11365
• Genome accession: NZ_CP121557
• Coordinates: 2407571..2408689 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain RAB9
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2402571..2413689

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTS96_RS11335 (MTS96_11335)	-	2403400..2404248 (+)	849	WP_000336548.1	class II glutamine amidotransferase	-
MTS96_RS11340 (MTS96_11340)	-	2404501..2404956 (+)	456	WP_000782976.1	bacteriohemerythrin	-
MTS96_RS11345 (MTS96_11345)	-	2405109..2406176 (+)	1068	WP_002019572.1	hypothetical protein	-
MTS96_RS11350 (MTS96_11350)	-	2406176..2406499 (+)	324	WP_000442587.1	RnfH family protein	-
MTS96_RS11355 (MTS96_11355)	bamE	2406554..2406952 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
MTS96_RS11360 (MTS96_11360)	fur	2407064..2407501 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
MTS96_RS11365 (MTS96_11365)	pilU	2407571..2408689 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MTS96_RS11370 (MTS96_11370)	pilT	2408717..2409754 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
MTS96_RS11375 (MTS96_11375)	-	2409881..2410573 (+)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=737403 MTS96_RS11365 WP_000347039.1 2407571..2408689(-) (pilU) [Acinetobacter baumannii strain RAB9]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=737403 MTS96_RS11365 WP_000347039.1 2407571..2408689(-) (pilU) [Acinetobacter baumannii strain RAB9]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395