Detailed information NTG0737213

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P9J63_RS04225
• Genome accession: NZ_CP121375
• Coordinates: 874756..875874 (-)
• Length: 372 a.a.
• NCBI ID: WP_032035964.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain LRT
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 869756..880874

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P9J63_RS04195 (P9J63_04195)	-	870586..871434 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
P9J63_RS04200 (P9J63_04200)	-	871687..872142 (+)	456	WP_000782976.1	bacteriohemerythrin	-
P9J63_RS04205 (P9J63_04205)	-	872295..873362 (+)	1068	WP_032035966.1	membrane protein	-
P9J63_RS04210 (P9J63_04210)	-	873362..873685 (+)	324	WP_005139957.1	RnfH family protein	-
P9J63_RS04215 (P9J63_04215)	bamE	873740..874138 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
P9J63_RS04220 (P9J63_04220)	fur	874250..874687 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
P9J63_RS04225 (P9J63_04225)	pilU	874756..875874 (-)	1119	WP_032035964.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P9J63_RS04230 (P9J63_04230)	pilT	875902..876939 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
P9J63_RS04235 (P9J63_04235)	-	877066..877758 (+)	693	WP_032035962.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41886.07 Da
• Isoelectric Point: 6.6215

>NTDB_id=737213 P9J63_RS04225 WP_032035964.1 874756..875874(-) (pilU) [Acinetobacter baumannii strain LRT]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDHQ

Nucleotide

• Length: 1119 bp

>NTDB_id=737213 P9J63_RS04225 WP_032035964.1 874756..875874(-) (pilU) [Acinetobacter baumannii strain LRT]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCACCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392