Detailed information NTG0737073

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P9J59_RS04220
• Genome accession: NZ_CP121365
• Coordinates: 873874..874992 (-)
• Length: 372 a.a.
• NCBI ID: WP_032035964.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain Ab_8_4
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 868874..879992

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P9J59_RS04190 (P9J59_04190)	-	869704..870552 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
P9J59_RS04195 (P9J59_04195)	-	870805..871260 (+)	456	WP_000782976.1	bacteriohemerythrin	-
P9J59_RS04200 (P9J59_04200)	-	871413..872480 (+)	1068	WP_032035966.1	membrane protein	-
P9J59_RS04205 (P9J59_04205)	-	872480..872803 (+)	324	WP_005139957.1	RnfH family protein	-
P9J59_RS04210 (P9J59_04210)	bamE	872858..873256 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
P9J59_RS04215 (P9J59_04215)	fur	873368..873805 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
P9J59_RS04220 (P9J59_04220)	pilU	873874..874992 (-)	1119	WP_032035964.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P9J59_RS04225 (P9J59_04225)	pilT	875020..876057 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
P9J59_RS04230 (P9J59_04230)	-	876184..876876 (+)	693	WP_032035962.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41886.07 Da
• Isoelectric Point: 6.6215

>NTDB_id=737073 P9J59_RS04220 WP_032035964.1 873874..874992(-) (pilU) [Acinetobacter baumannii strain Ab_8_4]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDHQ

Nucleotide

• Length: 1119 bp

>NTDB_id=737073 P9J59_RS04220 WP_032035964.1 873874..874992(-) (pilU) [Acinetobacter baumannii strain Ab_8_4]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCACCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392