Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   P9060_RS11485 Genome accession   NZ_CP121356
Coordinates   2288497..2289507 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain rl0044     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2283497..2294507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9060_RS11455 (P9060_11455) nudB 2285221..2285673 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  P9060_RS11460 (P9060_11460) yebC 2285702..2286442 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  P9060_RS11465 (P9060_11465) ruvC 2286477..2286998 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  P9060_RS11470 (P9060_11470) yebB 2287000..2287602 (-) 603 WP_001024932.1 YebB family permuted papain-like enzyme -
  P9060_RS11475 (P9060_11475) yobI 2287673..2287738 (+) 66 WP_010723105.1 stress response small protein YobI -
  P9060_RS11480 (P9060_11480) ruvA 2287877..2288488 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  P9060_RS11485 (P9060_11485) ruvB 2288497..2289507 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  P9060_RS11490 (P9060_11490) znuB 2289654..2290439 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  P9060_RS11495 (P9060_11495) znuC 2290436..2291191 (-) 756 WP_000202990.1 zinc ABC transporter ATP-binding protein ZnuC -
  P9060_RS11500 (P9060_11500) znuA 2291270..2292202 (+) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  P9060_RS11505 (P9060_11505) mepM 2292218..2293540 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=737008 P9060_RS11485 WP_000568519.1 2288497..2289507(+) (ruvB) [Escherichia coli strain rl0044]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=737008 P9060_RS11485 WP_000568519.1 2288497..2289507(+) (ruvB) [Escherichia coli strain rl0044]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494