Detailed information NTG0735406

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: P6F23_RS00415
• Genome accession: NZ_CP120953
• Coordinates: 65325..66323 (+)
• Length: 332 a.a.
• NCBI ID: WP_000196633.1
• Uniprot ID: Q3K3X8
• Organism: Streptococcus agalactiae strain GBSIR0001
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 60325..71323

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P6F23_RS00395 (P6F23_00395)	-	60871..61128 (+)	258	WP_000590468.1	hypothetical protein	-
P6F23_RS00400 (P6F23_00400)	-	61494..62597 (+)	1104	WP_001867169.1	DUF6287 domain-containing protein	-
P6F23_RS00405 (P6F23_00405)	purB	62683..63981 (+)	1299	WP_000893937.1	adenylosuccinate lyase	-
P6F23_RS00410 (P6F23_00410)	comR	64134..65036 (+)	903	WP_000912098.1	helix-turn-helix domain-containing protein	Regulator
P6F23_RS00415 (P6F23_00415)	ruvB	65325..66323 (+)	999	WP_000196633.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
P6F23_RS00420 (P6F23_00420)	-	66475..66912 (+)	438	WP_000754817.1	low molecular weight protein-tyrosine-phosphatase	-
P6F23_RS00425 (P6F23_00425)	-	66919..67299 (+)	381	WP_000787702.1	membrane protein	-
P6F23_RS00430 (P6F23_00430)	-	67296..69074 (+)	1779	WP_001220911.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37573.90 Da
• Isoelectric Point: 4.5053

>NTDB_id=735406 P6F23_RS00415 WP_000196633.1 65325..66323(+) (ruvB) [Streptococcus agalactiae strain GBSIR0001]
MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKSGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPRIANRLLKRVRDYAQIMGDGLID
DNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDK
AYEHLGYQRFDK

Nucleotide

• Length: 999 bp

>NTDB_id=735406 P6F23_RS00415 WP_000196633.1 65325..66323(+) (ruvB) [Streptococcus agalactiae strain GBSIR0001]
ATGACAAGATTTTTAGATAGTGATGCAATGGGTGACGAAGAATTGGTAGAACGTACACTTCGTCCGCAGTATTTAAGAGA
GTATATTGGACAAGATAAGGTTAAAGATCAGCTAAAAATATTTATTGAAGCTGCTAAATTGCGTGATGAGTCATTGGATC
ATGTGTTATTATTTGGCCCTCCTGGTTTAGGGAAAACAACCATGGCATTTGTAATTGCTAATGAGTTGGGTGTCAATCTC
AAACAAACATCAGGTCCCGCAATTGAAAAATCAGGGGATTTAGTAGCCATTTTAAATGATTTAGAACCAGGTGATGTTCT
TTTTATTGATGAAATTCATCGTATGCCGATGGCGGTTGAAGAGGTGCTCTATAGTGCAATGGAAGACTTTTATATTGACA
TTATGATTGGTGCAGGAGAAACTAGTAGAAGTGTTCATCTAGATTTGCCGCCCTTTACCTTAATTGGTGCAACGACACGT
GCAGGTATGTTATCTAATCCCTTACGTGCTCGCTTTGGTATTACAGGGCATATGGAGTATTATGAAGAAAATGATCTGAC
AGAAATTATTGAGCGTACAGCAGACATTTTTGAAATGAAAATTACTTATGAAGCTGCTTCTGAATTAGCGCGTCGCAGTC
GTGGAACGCCACGTATCGCTAACCGTTTATTGAAACGTGTTCGAGATTATGCTCAAATCATGGGAGATGGTTTGATAGAT
GACAATATTACAGATAAAGCATTAACGATGTTAGATGTTGATCACGAGGGGCTTGATTACGTCGATCAAAAAATCTTAAG
AACCATGATTGAAATGTATAATGGAGGTCCTGTTGGTTTAGGAACTCTATCCGTTAATATTGCTGAAGAACGAGATACTG
TTGAAGACATGTACGAACCTTATTTAATTCAAAAAGGTTTTATTATGCGTACCCGTACCGGTCGTGTAGCTACGGATAAG
GCATATGAACATTTAGGTTATCAGCGATTTGATAAATAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

3D structure

• AlphaFold DB: Q3K3X8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae TIGR4	90.06	100	0.901
ruvB	Streptococcus pneumoniae R6	89.759	100	0.898
ruvB	Streptococcus pneumoniae D39	89.759	100	0.898
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.819	99.699	0.596
ruvB	Helicobacter pylori 26695	53.074	93.072	0.494
ruvB	Synechocystis sp. PCC 6803	50.479	94.277	0.476