Detailed information NTG0735362

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: P7249_RS10560
• Genome accession: NZ_CP120944
• Coordinates: 2115843..2116853 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli O157:H7 str. EDL933
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2110843..2121853

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7249_RS10530	nudB	2112567..2113019 (+)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-
P7249_RS10535	yebC	2113048..2113788 (+)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
P7249_RS10540	ruvC	2113823..2114344 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
P7249_RS10545	yebB	2114346..2114948 (-)	603	WP_001024949.1	YebB family permuted papain-like enzyme	-
P7249_RS10550	yobI	2115019..2115084 (+)	66	WP_010723105.1	stress response small protein YobI	-
P7249_RS10555	ruvA	2115223..2115834 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
P7249_RS10560	ruvB	2115843..2116853 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
P7249_RS10565	znuB	2117099..2117884 (-)	786	WP_000571476.1	zinc ABC transporter permease subunit ZnuB	-
P7249_RS10570	znuC	2117881..2118636 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
P7249_RS10575	znuA	2118715..2119647 (+)	933	WP_001303192.1	zinc ABC transporter substrate-binding protein ZnuA	-
P7249_RS10580	mepM	2119663..2120985 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=735362 P7249_RS10560 WP_000568519.1 2115843..2116853(+) (ruvB) [Escherichia coli O157:H7 str. EDL933]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=735362 P7249_RS10560 WP_000568519.1 2115843..2116853(+) (ruvB) [Escherichia coli O157:H7 str. EDL933]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494