Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   P4G01_RS31110 Genome accession   NZ_CP120881
Coordinates   6461464..6462087 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain NY7583     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6456464..6467087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4G01_RS31090 (P4G01_31085) aroB 6457059..6458165 (-) 1107 WP_003114574.1 3-dehydroquinate synthase -
  P4G01_RS31095 (P4G01_31090) aroK 6458215..6458733 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P4G01_RS31100 (P4G01_31095) pilQ 6458745..6460889 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  P4G01_RS31105 (P4G01_31100) comL/pilP 6460943..6461467 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P4G01_RS31110 (P4G01_31105) pilO 6461464..6462087 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  P4G01_RS31115 (P4G01_31110) pilN 6462084..6462680 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  P4G01_RS31120 (P4G01_31115) pilM 6462680..6463744 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  P4G01_RS31125 (P4G01_31120) - 6463929..6466400 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=735025 P4G01_RS31110 WP_003103268.1 6461464..6462087(-) (pilO) [Pseudomonas aeruginosa strain NY7583]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=735025 P4G01_RS31110 WP_003103268.1 6461464..6462087(-) (pilO) [Pseudomonas aeruginosa strain NY7583]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401