Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   P4B34_RS02740 Genome accession   NZ_CP120859
Coordinates   556955..557578 (+) Length   207 a.a.
NCBI ID   WP_003095838.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY4383     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551955..562578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B34_RS02725 (P4B34_02730) - 552642..555113 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  P4B34_RS02730 (P4B34_02735) pilM 555298..556362 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  P4B34_RS02735 (P4B34_02740) pilN 556362..556958 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  P4B34_RS02740 (P4B34_02745) pilO 556955..557578 (+) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  P4B34_RS02745 (P4B34_02750) comL/pilP 557575..558099 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P4B34_RS02750 (P4B34_02755) pilQ 558153..560297 (+) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  P4B34_RS02755 (P4B34_02760) aroK 560309..560827 (+) 519 WP_003095833.1 shikimate kinase AroK -
  P4B34_RS02760 (P4B34_02765) aroB 560877..561983 (+) 1107 WP_003095831.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22805.23 Da        Isoelectric Point: 4.7787

>NTDB_id=734754 P4B34_RS02740 WP_003095838.1 556955..557578(+) (pilO) [Pseudomonas aeruginosa strain NY4383]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=734754 P4B34_RS02740 WP_003095838.1 556955..557578(+) (pilO) [Pseudomonas aeruginosa strain NY4383]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGGTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

44.211

91.787

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.684

91.787

0.401