Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PALA14_RS05455 Genome accession   NZ_CP106742
Coordinates   1157324..1159468 (+) Length   714 a.a.
NCBI ID   WP_023465329.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1152324..1164468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA14_RS05435 (PALA14_01071) pilM 1154469..1155533 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PALA14_RS05440 (PALA14_01072) pilN 1155533..1156129 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  PALA14_RS05445 (PALA14_01073) pilO 1156126..1156749 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  PALA14_RS05450 (PALA14_01074) pilP 1156746..1157270 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  PALA14_RS05455 (PALA14_01075) pilQ 1157324..1159468 (+) 2145 WP_023465329.1 type 4a pilus secretin PilQ Machinery gene
  PALA14_RS05460 (PALA14_01076) aroK 1159480..1159998 (+) 519 WP_003095833.1 shikimate kinase AroK -
  PALA14_RS05465 (PALA14_01077) aroB 1160048..1161154 (+) 1107 WP_003162482.1 3-dehydroquinate synthase -
  PALA14_RS05470 (PALA14_01078) - 1161161..1162810 (+) 1650 WP_003116139.1 AAA family ATPase -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77337.91 Da        Isoelectric Point: 5.2219

>NTDB_id=734233 PALA14_RS05455 WP_023465329.1 1157324..1159468(+) (pilQ) [Pseudomonas aeruginosa strain PALA14]
MNSGLSRLGIALLAAMLAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NGYGKDGNIGIKDEDGMNCGPIAGNCTFPTTGISKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFDRALNGVPPINKN
EVNAKILVNDGETIVIGGVFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVSDVKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=734233 PALA14_RS05455 WP_023465329.1 1157324..1159468(+) (pilQ) [Pseudomonas aeruginosa strain PALA14]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTAGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCATTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTACAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCTAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGCCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AACGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGCAACTGTAC
TTTCCCCACCACGGGAATCAGCAAGTCGCCGTCGCCGTTCGTCGACATCGGCGCCAAGGATGCCACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTACCCTACCAGGAGGCCAGCTC
CAGTGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGATGCGCCGGACTTCGATCGCGCACTGAACGGCGTACCGCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGCAACACCCAGAGCAAGTC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCTTACCTGGGGCGGCTGTTCCGGCGCGATACGGTCTCGGATGTGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(549-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

97.899

100

0.979
  pilQ Acinetobacter baumannii strain A118

39.814

100

0.419
  pilQ Acinetobacter baumannii D1279779

39.814

100

0.419
  comQ Acinetobacter baylyi ADP1

41.655

98.179

0.409