Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   N7921_RS12280 Genome accession   NZ_CP106652
Coordinates   2356709..2357002 (-) Length   97 a.a.
NCBI ID   WP_144484628.1    Uniprot ID   -
Organism   Bacillus safensis strain 33.4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2351709..2362002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7921_RS12230 (N7921_12230) sinR 2351877..2352212 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  N7921_RS12235 (N7921_12235) tasA 2352280..2353080 (-) 801 WP_111292280.1 biofilm matrix protein TasA -
  N7921_RS12240 (N7921_12240) sipW 2353135..2353707 (-) 573 WP_144484618.1 signal peptidase I SipW -
  N7921_RS12245 (N7921_12245) tapA 2353760..2354287 (-) 528 WP_095408998.1 amyloid fiber anchoring/assembly protein TapA -
  N7921_RS12250 (N7921_12250) - 2354587..2354865 (+) 279 WP_223251017.1 DUF3889 domain-containing protein -
  N7921_RS12255 (N7921_12255) - 2354899..2355093 (-) 195 WP_024718846.1 YqzE family protein -
  N7921_RS12260 (N7921_12260) - 2355153..2355536 (-) 384 WP_144484620.1 competence type IV pilus minor pilin ComGG -
  N7921_RS12265 (N7921_12265) comGF 2355533..2356060 (-) 528 WP_186437312.1 competence type IV pilus minor pilin ComGF -
  N7921_RS12270 (N7921_12270) comGE 2355972..2356286 (-) 315 WP_144484624.1 competence type IV pilus minor pilin ComGE -
  N7921_RS12275 (N7921_12275) comGD 2356270..2356716 (-) 447 WP_144484626.1 competence type IV pilus minor pilin ComGD -
  N7921_RS12280 (N7921_12280) comGC 2356709..2357002 (-) 294 WP_144484628.1 competence type IV pilus major pilin ComGC Machinery gene
  N7921_RS12285 (N7921_12285) comGB 2357019..2358059 (-) 1041 WP_144484630.1 competence type IV pilus assembly protein ComGB -
  N7921_RS12290 (N7921_12290) comGA 2358040..2359110 (-) 1071 WP_111292287.1 competence type IV pilus ATPase ComGA Machinery gene
  N7921_RS12295 (N7921_12295) - 2359288..2360163 (-) 876 WP_144484632.1 STAS domain-containing protein -
  N7921_RS12305 (N7921_12305) - 2360387..2360767 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  N7921_RS12310 (N7921_12310) - 2360980..2361222 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  N7921_RS12315 (N7921_12315) - 2361269..2361907 (-) 639 WP_076840972.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10709.66 Da        Isoelectric Point: 7.3837

>NTDB_id=733723 N7921_RS12280 WP_144484628.1 2356709..2357002(-) (comGC) [Bacillus safensis strain 33.4]
MNDKGFTLIEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGHVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=733723 N7921_RS12280 WP_144484628.1 2356709..2357002(-) (comGC) [Bacillus safensis strain 33.4]
ATGAATGATAAAGGATTTACCCTGATAGAAATGTTAATTGTCATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCTATTCAGGCAAAGGGCTGTGAAGGGCTCAAATCAATGGTCAGTACACAAGTGATGG
CATATGAATTGGAGCATGACGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGGTTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCCACGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

42.391

94.845

0.402
  comGC Staphylococcus aureus N315

42.391

94.845

0.402