Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   N7921_RS12290 Genome accession   NZ_CP106652
Coordinates   2358040..2359110 (-) Length   356 a.a.
NCBI ID   WP_111292287.1    Uniprot ID   -
Organism   Bacillus safensis strain 33.4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2353040..2364110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7921_RS12240 (N7921_12240) sipW 2353135..2353707 (-) 573 WP_144484618.1 signal peptidase I SipW -
  N7921_RS12245 (N7921_12245) tapA 2353760..2354287 (-) 528 WP_095408998.1 amyloid fiber anchoring/assembly protein TapA -
  N7921_RS12250 (N7921_12250) - 2354587..2354865 (+) 279 WP_223251017.1 DUF3889 domain-containing protein -
  N7921_RS12255 (N7921_12255) - 2354899..2355093 (-) 195 WP_024718846.1 YqzE family protein -
  N7921_RS12260 (N7921_12260) - 2355153..2355536 (-) 384 WP_144484620.1 competence type IV pilus minor pilin ComGG -
  N7921_RS12265 (N7921_12265) comGF 2355533..2356060 (-) 528 WP_186437312.1 competence type IV pilus minor pilin ComGF -
  N7921_RS12270 (N7921_12270) comGE 2355972..2356286 (-) 315 WP_144484624.1 competence type IV pilus minor pilin ComGE -
  N7921_RS12275 (N7921_12275) comGD 2356270..2356716 (-) 447 WP_144484626.1 competence type IV pilus minor pilin ComGD -
  N7921_RS12280 (N7921_12280) comGC 2356709..2357002 (-) 294 WP_144484628.1 competence type IV pilus major pilin ComGC Machinery gene
  N7921_RS12285 (N7921_12285) comGB 2357019..2358059 (-) 1041 WP_144484630.1 competence type IV pilus assembly protein ComGB -
  N7921_RS12290 (N7921_12290) comGA 2358040..2359110 (-) 1071 WP_111292287.1 competence type IV pilus ATPase ComGA Machinery gene
  N7921_RS12295 (N7921_12295) - 2359288..2360163 (-) 876 WP_144484632.1 STAS domain-containing protein -
  N7921_RS12305 (N7921_12305) - 2360387..2360767 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  N7921_RS12310 (N7921_12310) - 2360980..2361222 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  N7921_RS12315 (N7921_12315) - 2361269..2361907 (-) 639 WP_076840972.1 MBL fold metallo-hydrolase -
  N7921_RS12320 (N7921_12320) - 2362065..2362238 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  N7921_RS12325 (N7921_12325) - 2362264..2362578 (-) 315 WP_024427984.1 MTH1187 family thiamine-binding protein -
  N7921_RS12330 (N7921_12330) - 2362655..2363908 (-) 1254 WP_076840971.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40484.98 Da        Isoelectric Point: 9.4799

>NTDB_id=733724 N7921_RS12290 WP_111292287.1 2358040..2359110(-) (comGA) [Bacillus safensis strain 33.4]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELICPFCGETCQLYCKLNRPYRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=733724 N7921_RS12290 WP_111292287.1 2358040..2359110(-) (comGA) [Bacillus safensis strain 33.4]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCTTTCCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAACTAGATTATTATCATTTTTGA
AGCATTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTAATCCAGTTT
GCAAAAAAGAATTTTAATCGAAATATTATTACCCTTGAAGACCCTGTCGAAACGAGGAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTGG
GAGAAATAAGAGATGCGGAAACGGCTAGAACGGCGATTAGGGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTTCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTCGAACAAACCATGGTTGC
GATTGCTGCCCAGCGATTAATTGAGCTTATATGCCCTTTTTGCGGGGAAACATGTCAGCTTTACTGTAAATTAAATCGTC
CTTACAGACGAACAAATGTATTTGAATTGCTTTATGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCCAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTACAAAGATTAATTCGGAAAGGAGTGGCACTCGGTTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646