Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   N8E87_RS00475 Genome accession   NZ_CP104917
Coordinates   94993..96315 (+) Length   440 a.a.
NCBI ID   WP_264245254.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain AP-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 89993..101315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8E87_RS00455 (N8E87_00455) - 90095..92644 (-) 2550 WP_264245249.1 penicillin-binding protein 1A -
  N8E87_RS00460 (N8E87_00460) - 92803..93570 (+) 768 WP_264245250.1 hypothetical protein -
  N8E87_RS00465 (N8E87_00465) - 93600..94625 (+) 1026 WP_264245252.1 PilN domain-containing protein -
  N8E87_RS00470 (N8E87_00470) - 94625..94984 (+) 360 WP_017806828.1 hypothetical protein -
  N8E87_RS00475 (N8E87_00475) comE 94993..96315 (+) 1323 WP_264245254.1 type IV pilus secretin PilQ Machinery gene
  N8E87_RS00480 (N8E87_00480) aroK 96549..97076 (+) 528 WP_264245256.1 shikimate kinase AroK -
  N8E87_RS00485 (N8E87_00485) aroB 97093..98181 (+) 1089 WP_264245258.1 3-dehydroquinate synthase -
  N8E87_RS00490 (N8E87_00490) - 98187..99077 (+) 891 WP_264245259.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  N8E87_RS00495 (N8E87_00495) - 99049..99354 (-) 306 WP_035688115.1 DciA family protein -
  N8E87_RS00500 (N8E87_00500) secM 99460..99771 (+) 312 WP_026138577.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 48578.53 Da        Isoelectric Point: 6.1197

>NTDB_id=733489 N8E87_RS00475 WP_264245254.1 94993..96315(+) (comE) [Avibacterium paragallinarum strain AP-2]
MKLPNIIKNILLFFLILSYPFRQVNASDENNTFSVHLKQAPLVDTVQYLAGYQKINLVIDDELSGSISLQLQNTTLLQLL
KSIAEIKHLRFWENNGIYYLSQSQATNQENVVLNSSTDIATSADMLITSIKLHYAKASEVMKALTSGNGSLLTANGSISF
DERNNLLIIQDEAKKIKQIRKLVADMDKPIQQIIIEARIVTISDESLKELGVRWGIFSPSDISHSVSGSLNANGFSNITD
NLNINFATSSNPAGSVALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKSASIKQGTEIPYVVNNGKNDTQSIE
FREAVLGLEVTPHLLKHNGILLDLVVSQNAPGGRVSYGENEVISIDKQEINTQVFAQDGETIVLGGIFHDTITKALDKVP
ILGDIPGVKRLFSKEVERHQKRELVIFVTPRLIRSGTVEE

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=733489 N8E87_RS00475 WP_264245254.1 94993..96315(+) (comE) [Avibacterium paragallinarum strain AP-2]
ATGAAGTTACCAAATATAATAAAGAATATTCTTTTATTTTTTCTTATTCTATCCTATCCATTTAGACAGGTTAATGCAAG
TGACGAAAATAATACATTCTCTGTTCATTTAAAGCAAGCTCCATTAGTAGATACTGTACAATATTTAGCTGGGTATCAAA
AAATCAATTTAGTCATAGATGATGAATTGTCTGGTTCAATATCTTTGCAATTGCAAAATACAACATTACTTCAATTATTA
AAATCTATCGCTGAAATAAAACATTTGCGGTTTTGGGAAAATAATGGGATATATTATTTAAGTCAGTCGCAAGCAACTAA
TCAAGAAAATGTAGTGTTAAATTCATCAACAGACATAGCAACATCAGCAGATATGTTAATAACTTCAATAAAGTTGCACT
ATGCCAAAGCATCCGAGGTAATGAAAGCCCTCACTTCAGGGAATGGCTCTTTATTAACAGCAAATGGAAGTATAAGTTTT
GATGAACGCAATAATTTACTCATTATCCAAGATGAAGCTAAAAAAATAAAACAAATTAGGAAATTGGTAGCGGATATGGA
TAAACCCATTCAGCAAATTATTATTGAAGCTCGTATTGTCACAATTAGCGATGAAAGTTTAAAAGAATTGGGCGTTAGAT
GGGGAATTTTTTCTCCCTCAGATATAAGCCATAGCGTTTCTGGAAGTTTAAATGCAAATGGATTCTCAAATATTACGGAT
AATCTAAATATTAATTTTGCGACTAGTTCAAATCCTGCAGGTTCGGTAGCTTTGCAAGTAGCTAAAATTAATGGAAGGTT
ATTAGATTTAGAGTTGACAGCATTAGAAAGAGAAAATAACGTTGAAATTATTGCGAGTCCACGATTATTGACCACAAATA
AGAAAAGTGCAAGTATCAAGCAAGGTACAGAAATCCCCTATGTAGTGAATAATGGAAAAAATGATACGCAGTCTATTGAA
TTCCGAGAAGCTGTACTTGGGCTTGAAGTTACACCTCATTTATTAAAGCATAACGGTATTTTACTTGATCTTGTTGTTAG
CCAGAATGCACCTGGAGGACGAGTATCTTATGGAGAAAATGAGGTGATTTCTATTGATAAACAGGAAATTAATACGCAAG
TCTTTGCACAAGACGGCGAAACGATTGTATTGGGCGGAATTTTCCACGATACGATTACTAAAGCACTTGATAAAGTACCA
ATTTTAGGAGACATACCTGGAGTAAAAAGGCTATTTAGTAAGGAGGTCGAGAGACATCAAAAGAGGGAACTCGTTATTTT
TGTTACACCACGCTTAATTCGTAGTGGTACAGTAGAGGAATGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(129-192)

Bacterial type II and III secretion system protein (Secretin)

(274-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

64.419

97.727

0.63
  comE Haemophilus influenzae 86-028NP

65.707

94.773

0.623
  comE Glaesserella parasuis strain SC1401

49.649

97.045

0.482
  pilQ Vibrio campbellii strain DS40M4

40

96.591

0.386
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.288

94.773

0.382
  pilQ Vibrio cholerae strain A1552

40.288

94.773

0.382