Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   P5661_RS14795 Genome accession   NZ_CP120600
Coordinates   2773249..2774253 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain PRO112     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2768249..2779253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5661_RS14765 (P5661_14765) yrbG 2769223..2769879 (-) 657 WP_015483509.1 DUF421 domain-containing protein -
  P5661_RS14770 (P5661_14770) - 2770077..2770466 (+) 390 WP_004398667.1 TIGR04086 family membrane protein -
  P5661_RS14775 (P5661_14775) yajC 2770523..2770789 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  P5661_RS14780 (P5661_14780) tgt 2770826..2771971 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  P5661_RS14785 (P5661_14785) queA 2771998..2773026 (-) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  P5661_RS14790 (P5661_14790) yrzS 2773056..2773256 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  P5661_RS14795 (P5661_14795) ruvB 2773249..2774253 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  P5661_RS14800 (P5661_14800) ruvA 2774264..2774869 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  P5661_RS14805 (P5661_14805) bofC 2775008..2775520 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  P5661_RS14810 (P5661_14810) csbX 2775568..2776875 (-) 1308 WP_015483512.1 MFS transporter -
  P5661_RS14815 (P5661_14815) iolG 2776946..2777971 (-) 1026 WP_277708863.1 inositol 2-dehydrogenase -
  P5661_RS14820 (P5661_14820) yrzF 2778209..2778856 (+) 648 WP_049141074.1 phosphotransferase -
  P5661_RS14825 (P5661_14825) - 2778902..2779024 (-) 123 WP_003229707.1 hypothetical protein -
  P5661_RS14830 (P5661_14830) - 2779109..2779183 (+) 75 Protein_2871 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=733010 P5661_RS14795 WP_003229718.1 2773249..2774253(-) (ruvB) [Bacillus subtilis strain PRO112]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=733010 P5661_RS14795 WP_003229718.1 2773249..2774253(-) (ruvB) [Bacillus subtilis strain PRO112]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTAGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCGCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518