Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PALA8_RS25465 Genome accession   NZ_CP104869
Coordinates   5483494..5484831 (+) Length   445 a.a.
NCBI ID   WP_003110427.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA8     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5478494..5489831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA8_RS25440 (PALA8_05065) pgeF 5478637..5479365 (-) 729 WP_003134928.1 peptidoglycan editing factor PgeF -
  PALA8_RS25445 (PALA8_05066) rluD 5479362..5480324 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PALA8_RS25450 (PALA8_05067) - 5480470..5481495 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  PALA8_RS25455 (PALA8_05068) - 5481652..5481897 (+) 246 WP_014603563.1 PP0621 family protein -
  PALA8_RS25460 (PALA8_05069) pilS 5481887..5483479 (+) 1593 WP_003110428.1 two-component system sensor histidine kinase PilS Regulator
  PALA8_RS25465 (PALA8_05070) pilR 5483494..5484831 (+) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  PALA8_RS25470 (PALA8_05071) thiO 5484877..5485971 (-) 1095 WP_003134931.1 glycine oxidase ThiO -
  PALA8_RS25475 (PALA8_05072) - 5486106..5486615 (+) 510 WP_003134932.1 GspH/FimT family pseudopilin -
  PALA8_RS25480 (PALA8_05073) - 5486721..5487227 (+) 507 WP_003134935.1 GspH/FimT family pseudopilin -
  PALA8_RS25485 (PALA8_05074) pilV 5487218..5487775 (+) 558 WP_003110422.1 type 4a pilus minor pilin PilV Machinery gene
  PALA8_RS25490 (PALA8_05075) pilW 5487772..5488596 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  PALA8_RS25495 (PALA8_05076) pilX 5488593..5489180 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49723.03 Da        Isoelectric Point: 5.8638

>NTDB_id=733005 PALA8_RS25465 WP_003110427.1 5483494..5484831(+) (pilR) [Pseudomonas aeruginosa strain PALA8]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLADRI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=733005 PALA8_RS25465 WP_003110427.1 5483494..5484831(+) (pilR) [Pseudomonas aeruginosa strain PALA8]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGATCGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCAGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCACTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384