Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   P5709_RS06300 Genome accession   NZ_CP120557
Coordinates   1228399..1229409 (-) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain B771     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1223399..1234409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5709_RS06280 (P5709_06280) mepM 1224172..1225494 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  P5709_RS06285 (P5709_06285) znuA 1225510..1226442 (-) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  P5709_RS06290 (P5709_06290) znuC 1226521..1227276 (+) 756 WP_000202990.1 zinc ABC transporter ATP-binding protein ZnuC -
  P5709_RS06295 (P5709_06295) znuB 1227273..1228058 (+) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  P5709_RS06300 (P5709_06300) ruvB 1228399..1229409 (-) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  P5709_RS06305 (P5709_06305) ruvA 1229418..1230029 (-) 612 WP_000580332.1 Holliday junction branch migration protein RuvA -
  P5709_RS06310 (P5709_06310) yobI 1230168..1230233 (-) 66 WP_072094247.1 stress response small protein YobI -
  P5709_RS06315 (P5709_06315) yebB 1230304..1230906 (+) 603 WP_001024904.1 YebB family permuted papain-like enzyme -
  P5709_RS06320 (P5709_06320) ruvC 1230908..1231429 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  P5709_RS06325 (P5709_06325) yebC 1231464..1232204 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  P5709_RS06330 (P5709_06330) nudB 1232233..1232685 (-) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=732291 P5709_RS06300 WP_000568519.1 1228399..1229409(-) (ruvB) [Escherichia coli strain B771]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=732291 P5709_RS06300 WP_000568519.1 1228399..1229409(-) (ruvB) [Escherichia coli strain B771]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGGATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGGGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494