Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   P5710_RS10490 Genome accession   NZ_CP120549
Coordinates   2230101..2231111 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain B1172     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2225101..2236111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5710_RS10460 (P5710_10460) nudB 2226825..2227277 (+) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -
  P5710_RS10465 (P5710_10465) yebC 2227306..2228046 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  P5710_RS10470 (P5710_10470) ruvC 2228081..2228602 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  P5710_RS10475 (P5710_10475) yebB 2228604..2229206 (-) 603 WP_001024939.1 YebB family permuted papain-like enzyme -
  P5710_RS10480 (P5710_10480) yobI 2229277..2229342 (+) 66 WP_072093883.1 stress response small protein YobI -
  P5710_RS10485 (P5710_10485) ruvA 2229481..2230092 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  P5710_RS10490 (P5710_10490) ruvB 2230101..2231111 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  P5710_RS10495 (P5710_10495) znuB 2231256..2232041 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  P5710_RS10500 (P5710_10500) znuC 2232038..2232793 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  P5710_RS10505 (P5710_10505) znuA 2232872..2233804 (+) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -
  P5710_RS10510 (P5710_10510) mepM 2233820..2235142 (+) 1323 WP_001518672.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=732236 P5710_RS10490 WP_000568519.1 2230101..2231111(+) (ruvB) [Escherichia coli strain B1172]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=732236 P5710_RS10490 WP_000568519.1 2230101..2231111(+) (ruvB) [Escherichia coli strain B1172]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATACATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494