Detailed information NTG0730720

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: N5O87_RS19575
• Genome accession: NZ_CP104582
• Coordinates: 4067357..4068421 (-)
• Length: 354 a.a.
• NCBI ID: WP_037004588.1
• Uniprot ID: -
• Organism: Pseudomonas sp. GD03919
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4062357..4073421

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5O87_RS19550 (N5O87_19540)	aroK	4062950..4063468 (-)	519	WP_084341405.1	shikimate kinase AroK	-
N5O87_RS19555 (N5O87_19545)	pilQ	4063474..4065588 (-)	2115	WP_279533202.1	type IV pilus secretin PilQ	Machinery gene
N5O87_RS19560 (N5O87_19550)	pilP	4065644..4066168 (-)	525	WP_279531406.1	type 4a pilus biogenesis lipoprotein PilP	-
N5O87_RS19565 (N5O87_19555)	pilO	4066168..4066788 (-)	621	WP_279531407.1	type 4a pilus biogenesis protein PilO	-
N5O87_RS19570 (N5O87_19560)	-	4066785..4067357 (-)	573	WP_279531408.1	PilN domain-containing protein	-
N5O87_RS19575 (N5O87_19565)	pilM	4067357..4068421 (-)	1065	WP_037004588.1	pilus assembly protein PilM	Machinery gene
N5O87_RS19580 (N5O87_19570)	-	4068603..4071014 (+)	2412	WP_279531409.1	penicillin-binding protein 1A	-
N5O87_RS19585 (N5O87_19575)	-	4071105..4072373 (-)	1269	WP_147812416.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37827.22 Da
• Isoelectric Point: 4.4647

>NTDB_id=730720 N5O87_RS19575 WP_037004588.1 4067357..4068421(-) (pilM) [Pseudomonas sp. GD03919]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLEAQLGAGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=730720 N5O87_RS19575 WP_037004588.1 4067357..4068421(-) (pilM) [Pseudomonas sp. GD03919]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCCTATGCCGTCGAGCCACTTCCGCCCAATGCAGTGGTCGAGAAGAACA
TTGCCGAGTTGGAGGGGGTCGGTCAGGCACTTTCTCGCGTTTTGGCCAAGGCCAAGAGCGGTGTCAAGACTGCGGCCGTT
GCCGTTGCTGGTTCGGCCGTGATCACCAAAACCATCGAAATGGAAGCCGGGCTTTCCGAGGACGATCTGGAGAATCAGCT
CAAGATCGAGGCAGATCAGTACATTCCCTACCCGCTGGAAGAAGTGGCCATCGATTTCGAGGTTCAGGGGCCGGCGCCGC
GTAATCCCGAGCGTGTCGAGGTGTTGCTGGCGGCATGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGCTTGCGCTT
GCGGGGCTTACCGCGAAAGTGGTCGACGTCGAAGCCTATGCCCTCGAACGTGCCTACGGTCTGCTGGAGGCGCAACTGGG
CGCTGGTCATGACGAGTTGACCGTCGCTGTAGTGGATATCGGTGCGACCATGACTACGTTGAGCGTCCTGCACAACGGTC
GCACCATCTACACCCGCGAGCAGCTCTTCGGCGGCAAGCAACTGACCGAGGAAATCCAGCGTCGCTATGGTCTGTCCGTG
GAGGAAGCCGGGCTTGCCAAGAAGCAAGGTGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAGCCGTTCAAAGAAGC
TGTTGTTCAGCAGGTTTCCCGCTCGCTGCAGTTTTTCTTCGCTGCTGGGCAGTTCAACGATGTCGACTACATACTCCTCG
CCGGCGGCACTGCATCGATCCCTGATCTCGATCGGTTGATCCAGCAAAAAATCGGTACGCAGACCCTGGTGGCCAACCCC
TTTGCCGATATGGCGCTCAGTAGCAAGGTCAATGCCGGTGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.497	100	0.565
comM	Acinetobacter nosocomialis M2	56.497	100	0.565
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	45.198	100	0.452