Detailed information NTG0730710

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N5O87_RS11060
• Genome accession: NZ_CP104582
• Coordinates: 2293854..2295074 (+)
• Length: 406 a.a.
• NCBI ID: WP_187272867.1
• Uniprot ID: -
• Organism: Pseudomonas sp. GD03919
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2288854..2300074

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5O87_RS11040 (N5O87_11045)	-	2290037..2292490 (+)	2454	WP_279533093.1	YbcC family protein	-
N5O87_RS11045 (N5O87_11050)	-	2292537..2292802 (-)	266	Protein_2169	hypothetical protein	-
N5O87_RS11050 (N5O87_11055)	-	2292943..2293259 (+)	317	Protein_2170	LysR family transcriptional regulator	-
N5O87_RS11055 (N5O87_11060)	-	2293427..2293705 (-)	279	WP_017362973.1	peptidylprolyl isomerase	-
N5O87_RS11060 (N5O87_11065)	pilU	2293854..2295074 (+)	1221	WP_187272867.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N5O87_RS11065 (N5O87_11070)	-	2295116..2295727 (-)	612	WP_279533094.1	substrate-binding periplasmic protein	-
N5O87_RS11070 (N5O87_11075)	-	2295780..2295923 (-)	144	WP_279533095.1	hypothetical protein	-
N5O87_RS11075 (N5O87_11080)	-	2295929..2296882 (-)	954	WP_279533096.1	sensor domain-containing diguanylate cyclase	-
N5O87_RS11080 (N5O87_11085)	-	2296869..2297696 (-)	828	WP_279533097.1	alpha/beta fold hydrolase	-
N5O87_RS11085 (N5O87_11090)	-	2297808..2299763 (-)	1956	WP_279533098.1	acetoacetate--CoA ligase	-

Sequence

Protein

• Length: 406 a.a.
• Molecular weight: 44687.05 Da
• Isoelectric Point: 6.3583

>NTDB_id=730710 N5O87_RS11060 WP_187272867.1 2293854..2295074(+) (pilU) [Pseudomonas sp. GD03919]
MDLHAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSQEPMKAGDVAAIADSIMDSAQREEFERELEMNLAISLTGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLGTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTLDGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLHRDNPANARPAPAVAAAPAAQPVAAKSAAAGDWGLELKLEEIEQEPLPED
PGRQGI

Nucleotide

• Length: 1221 bp

>NTDB_id=730710 N5O87_RS11060 WP_187272867.1 2293854..2295074(+) (pilU) [Pseudomonas sp. GD03919]
ATGGATCTCCACGCAATGCTGAAAATCCTGTCCAGTCAGGATGGCTCCGATCTGTATCTGTCCACTGGCGCGCCGCCTTG
CGCCAAGTTCAACGGCGTGCTCAAGGCACTGAGCCAGGAGCCGATGAAAGCCGGCGATGTGGCGGCGATTGCCGACTCGA
TCATGGACAGTGCCCAGCGTGAGGAATTCGAGCGCGAACTGGAGATGAACCTGGCGATATCGCTGACCGGGGTCGGACGT
TTCCGCATCAACATCTTCAAGCAGCGTAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGATATTCCGCGTTTCGA
AGACCTCAAACTGCCCGAAGTGCTGCTCGGCACGGTGATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAGTCGACCTCGCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGTCACATCATCACCATCGAAGAC
CCGGTGGAGTATGTGCACCGGCACAAGAAATCGATCATCAACCAGCGCGAGGTGGGCGTGGATACCCGCAGCTTTCACGC
GGCGCTGAAGAACACCCTGCGTCAGGCGCCGGATGTGATCCTGATTGGCGAAATTCGCGACCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCCGATACCGGACACCTGGCGATTTCCACGCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGACCGCCGCCCACAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTCGTTTCCCAGCG
CCTGGTCAAGACGCTTGACGGCAAGCGCCGCGCCGCAGTTGAGGTGATGCTGGGCACGCCGACGGTGCGCGACCTGATCA
AGCGCAACGAGTTTTCCGAGCTGAAGGAAATCATGGAGAAGTCCAAGAATCTCGGCATGCAGACTTTCGACCAGGCGCTG
ATCGATCTGGTGCACGAAGGTGCAATCGACGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTGAA
ACTCAAGCTGCATCGAGACAACCCGGCCAACGCACGGCCTGCACCTGCAGTAGCAGCTGCCCCTGCGGCGCAGCCCGTTG
CAGCGAAAAGTGCTGCAGCCGGTGATTGGGGCCTGGAACTGAAGCTGGAGGAGATCGAGCAGGAGCCGTTGCCAGAAGAT
CCGGGGCGGCAGGGTATTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	86.453	0.488
pilU	Acinetobacter baylyi ADP1	51.989	86.7	0.451
pilU	Vibrio cholerae strain A1552	51.558	86.946	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.44	96.798	0.382