Detailed information NTG0730671

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N5O82_RS21060
• Genome accession: NZ_CP104580
• Coordinates: 4468047..4469192 (-)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain GD04120
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4463047..4474192

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5O82_RS21035 (N5O82_21030)	-	4463124..4464140 (+)	1017	WP_048330150.1	aspartate carbamoyltransferase catalytic subunit	-
N5O82_RS21040 (N5O82_21035)	-	4464140..4465414 (+)	1275	WP_053528279.1	dihydroorotase	-
N5O82_RS21045 (N5O82_21040)	-	4465472..4465867 (-)	396	WP_011914966.1	TM2 domain-containing protein	-
N5O82_RS21050 (N5O82_21045)	-	4466003..4467217 (+)	1215	WP_053528278.1	putative DNA modification/repair radical SAM protein	-
N5O82_RS21055 (N5O82_21050)	-	4467217..4468050 (+)	834	WP_049325486.1	TIGR03915 family putative DNA repair protein	-
N5O82_RS21060 (N5O82_21055)	pilU	4468047..4469192 (-)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N5O82_RS21065 (N5O82_21060)	pilT	4469252..4470286 (-)	1035	WP_011914970.1	type IV pilus twitching motility protein PilT	Machinery gene
N5O82_RS21070 (N5O82_21065)	-	4470353..4471042 (+)	690	WP_125885947.1	YggS family pyridoxal phosphate-dependent enzyme	-
N5O82_RS21075 (N5O82_21070)	proC	4471053..4471877 (+)	825	WP_049340282.1	pyrroline-5-carboxylate reductase	-
N5O82_RS21080 (N5O82_21075)	-	4471887..4472477 (+)	591	WP_014598006.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=730671 N5O82_RS21060 WP_019405534.1 4468047..4469192(-) (pilU) [Stutzerimonas stutzeri strain GD04120]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=730671 N5O82_RS21060 WP_019405534.1 4468047..4469192(-) (pilU) [Stutzerimonas stutzeri strain GD04120]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCATGAATGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCGACGTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTCATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGTGCAGGGCGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391