NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	N5O81_RS12790	Genome accession	NZ_CP104503
Coordinates	2634770..2635894 (-)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	E2QFE2
Organism	Escherichia coli strain E1
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 2629770..2640894

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5O81_RS12760 (N5O81_12760)	yrdA	2631570..2632124 (+)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-
N5O81_RS12765 (N5O81_12765)	yrdB	2632100..2632357 (-)	258	WP_001070567.1	DUF1488 domain-containing protein	-
N5O81_RS12770 (N5O81_12770)	aroE	2632354..2633172 (-)	819	WP_000451230.1	shikimate dehydrogenase	-
N5O81_RS12775 (N5O81_12775)	tsaC	2633177..2633749 (-)	573	WP_096849349.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
N5O81_RS12780 (N5O81_12780)	yrdD	2633754..2634296 (-)	543	WP_001312137.1	type I DNA topoisomerase	-
N5O81_RS12785 (N5O81_12785)	smg	2634325..2634798 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
N5O81_RS12790 (N5O81_12790)	dprA	2634770..2635894 (-)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
N5O81_RS12795 (N5O81_12795)	def	2636024..2636533 (+)	510	WP_000114984.1	peptide deformylase	-
N5O81_RS12800 (N5O81_12800)	fmt	2636548..2637495 (+)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
N5O81_RS12805 (N5O81_12805)	rsmB	2637547..2638836 (+)	1290	WP_000744767.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
N5O81_RS12810 (N5O81_12810)	trkA	2638858..2640234 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
N5O81_RS12815 (N5O81_12815)	mscL	2640364..2640777 (+)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=730115 N5O81_RS12790 WP_000228517.1 2634770..2635894(-) (dprA) [Escherichia coli strain E1]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=730115 N5O81_RS12790 WP_000228517.1 2634770..2635894(-) (dprA) [Escherichia coli strain E1]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	E2QFE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0730115