Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   Vt282_RS01045 Genome accession   NZ_AP019649
Coordinates   224238..224828 (+) Length   196 a.a.
NCBI ID   WP_162047334.1    Uniprot ID   -
Organism   Vibrio taketomensis strain C4III282     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219238..229828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Vt282_RS01030 - 219998..222547 (-) 2550 WP_162062342.1 penicillin-binding protein 1A -
  Vt282_RS01035 pilM 222686..223678 (+) 993 WP_162062343.1 type IV pilus assembly protein PilM -
  Vt282_RS01040 - 223668..224245 (+) 578 Protein_213 PilN domain-containing protein -
  Vt282_RS01045 pilO 224238..224828 (+) 591 WP_162047334.1 type 4a pilus biogenesis protein PilO Machinery gene
  Vt282_RS01050 pilP 224818..225333 (+) 516 WP_162062344.1 pilus assembly protein PilP Machinery gene
  Vt282_RS01055 pilQ 225352..227130 (+) 1779 WP_162062345.1 type IV pilus secretin PilQ family protein Machinery gene
  Vt282_RS01060 aroK 227294..227812 (+) 519 WP_162047331.1 shikimate kinase AroK -
  Vt282_RS01065 aroB 227843..228931 (+) 1089 WP_162062346.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22730.97 Da        Isoelectric Point: 4.8927

>NTDB_id=72995 Vt282_RS01045 WP_162047334.1 224238..224828(+) (pilO) [Vibrio taketomensis strain C4III282]
MAKYTELDVEDIVHWPVLAQVAVLAVLVAVLQAVGFWWYLDHKIEDLDQLKQQEIQLKQTVQIKANKVAALPQLQAQLDE
LSQRYDYLSRQLPVQKELATMLASVNEQGLKNSLTFTRIDWGEKQNQQFLYRLPLNIELTGDYNDIGEFSQAIAGLPRII
NFLDVEWHRVSLESSTLHFRVRAYTYQFKPEVKDEG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=72995 Vt282_RS01045 WP_162047334.1 224238..224828(+) (pilO) [Vibrio taketomensis strain C4III282]
GTGGCTAAGTATACTGAGCTCGATGTCGAAGACATTGTTCATTGGCCCGTCTTGGCTCAAGTTGCGGTACTTGCAGTATT
AGTTGCAGTGCTGCAAGCGGTGGGTTTTTGGTGGTATCTCGATCATAAAATTGAAGATCTCGATCAGCTTAAGCAACAAG
AAATTCAGCTTAAGCAGACGGTACAAATCAAAGCCAACAAAGTTGCCGCATTACCTCAACTGCAAGCTCAACTTGATGAG
TTATCGCAACGCTACGATTACTTATCTCGCCAGCTTCCTGTGCAAAAAGAGTTAGCGACGATGTTGGCATCTGTAAACGA
ACAAGGTTTGAAAAATAGCCTGACTTTCACTCGTATCGACTGGGGTGAAAAGCAAAATCAGCAGTTCCTATATCGTTTAC
CGCTAAATATCGAATTGACTGGTGATTACAATGATATTGGTGAGTTCTCACAAGCTATCGCAGGGCTACCAAGGATCATC
AACTTTTTAGACGTGGAATGGCATCGCGTTAGTTTAGAGAGTAGCACGCTGCACTTTAGGGTTCGCGCTTATACCTACCA
GTTTAAACCGGAGGTGAAAGATGAAGGCTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

66.667

99.49

0.663
  pilO Vibrio campbellii strain DS40M4

61.579

96.939

0.597

Multiple sequence alignment