Detailed information NTG0729337

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: N4R43_RS07935
• Genome accession: NZ_CP104388
• Coordinates: 1528676..1529998 (-)
• Length: 440 a.a.
• NCBI ID: WP_058223060.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain LJL7m20
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1523676..1534998

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4R43_RS07905 (N4R43_07910)	-	1524820..1525788 (+)	969	WP_003130649.1	PhoH family protein	-
N4R43_RS07910 (N4R43_07915)	-	1525846..1526325 (+)	480	WP_058223061.1	NUDIX hydrolase	-
N4R43_RS07915 (N4R43_07920)	ybeY	1526477..1526965 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
N4R43_RS07920 (N4R43_07925)	-	1526949..1527404 (+)	456	WP_003130653.1	diacylglycerol kinase family protein	-
N4R43_RS07925 (N4R43_07930)	-	1527418..1527996 (+)	579	WP_023189343.1	nucleotidyltransferase family protein	-
N4R43_RS07930 (N4R43_07935)	comFC	1528029..1528433 (-)	405	WP_021722514.1	ComF family protein	Machinery gene
N4R43_RS07935 (N4R43_07940)	comFA	1528676..1529998 (-)	1323	WP_058223060.1	DEAD/DEAH box helicase	Machinery gene
N4R43_RS07940 (N4R43_07945)	-	1530055..1530684 (+)	630	WP_003130660.1	YigZ family protein	-
N4R43_RS07945 (N4R43_07950)	-	1530818..1531315 (+)	498	WP_023189340.1	VanZ family protein	-
N4R43_RS07950 (N4R43_07955)	-	1531363..1532382 (+)	1020	WP_012897709.1	DUF475 domain-containing protein	-
N4R43_RS07955 (N4R43_07960)	-	1532497..1533243 (+)	747	WP_058223057.1	bifunctional metallophosphatase/5'-nucleotidase	-
N4R43_RS07960 (N4R43_07965)	-	1533535..1534182 (+)	648	WP_058223056.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50300.85 Da
• Isoelectric Point: 10.1066

>NTDB_id=729337 N4R43_RS07935 WP_058223060.1 1528676..1529998(-) (comFA) [Lactococcus lactis strain LJL7m20]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHHQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLVGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=729337 N4R43_RS07935 WP_058223060.1 1528676..1529998(-) (comFA) [Lactococcus lactis strain LJL7m20]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
ATCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGTAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

• Type III restriction enzyme, res subunit (ResIII) (114-233)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	73.75	90.909	0.67
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	37.029	100	0.38