Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5B55_RS02355 Genome accession   NZ_CP104381
Coordinates   502003..502566 (+) Length   187 a.a.
NCBI ID   WP_304539008.1    Uniprot ID   -
Organism   Ralstonia pickettii strain MCR     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497003..507566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5B55_RS02330 (N5B55_02330) recX 497379..497858 (+) 480 WP_065856791.1 recombination regulator RecX -
  N5B55_RS02335 (N5B55_02335) - 498150..498776 (+) 627 WP_304539005.1 DUF2889 domain-containing protein -
  N5B55_RS02340 (N5B55_02340) sucC 498843..500009 (+) 1167 WP_004635396.1 ADP-forming succinate--CoA ligase subunit beta -
  N5B55_RS02345 (N5B55_02345) sucD 500098..500979 (+) 882 WP_304539006.1 succinate--CoA ligase subunit alpha -
  N5B55_RS02350 (N5B55_02350) - 501128..501823 (+) 696 WP_304539007.1 TerC family protein -
  N5B55_RS02355 (N5B55_02355) pilA 502003..502566 (+) 564 WP_304539008.1 pilin Machinery gene
  N5B55_RS02360 (N5B55_02360) tfpZ 502666..503442 (+) 777 WP_304539009.1 TfpX/TfpZ family type IV pilin accessory protein -
  N5B55_RS02365 (N5B55_02365) - 503565..505298 (+) 1734 WP_304539010.1 PglL family O-oligosaccharyltransferase -
  N5B55_RS02370 (N5B55_02370) - 505470..505952 (+) 483 WP_304539011.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  N5B55_RS02375 (N5B55_02375) moaC 505998..506474 (-) 477 WP_304539012.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 18987.83 Da        Isoelectric Point: 9.1973

>NTDB_id=729250 N5B55_RS02355 WP_304539008.1 502003..502566(+) (pilA) [Ralstonia pickettii strain MCR]
MKSMRLNKRVQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRAKVSDGLVLASGLKSVVADNAANGTPDASGGFFASMP
TSQDGTGLCNAKGTCSLNGGKGLGNNLTSIIGTTTDGHIQITYSSSVAPANANTVELWPTAAGAGLTVGTPPATSLVWTC
YTANKKGVDGVTPGATLLAKFAPPQCR

Nucleotide


Download         Length: 564 bp        

>NTDB_id=729250 N5B55_RS02355 WP_304539008.1 502003..502566(+) (pilA) [Ralstonia pickettii strain MCR]
ATGAAGTCGATGCGACTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATTATCGG
TATTCTGGCTGCCATCGCGCTGCCGGCTTATCAGGATTACACCGTTCGCGCGAAGGTATCGGATGGTCTGGTATTGGCAT
CCGGTCTGAAGTCTGTCGTGGCAGACAACGCTGCTAACGGCACTCCCGATGCTAGCGGCGGTTTCTTTGCGAGCATGCCG
ACTAGTCAGGATGGCACTGGGCTGTGCAACGCCAAGGGCACCTGTTCGCTGAATGGCGGTAAGGGCCTCGGTAACAATTT
GACTTCTATTATTGGCACCACTACAGATGGGCATATTCAGATCACCTATTCCAGTTCGGTCGCGCCGGCTAACGCCAATA
CTGTGGAACTGTGGCCGACCGCAGCCGGTGCTGGGTTGACGGTTGGCACTCCGCCTGCCACTAGCCTTGTTTGGACCTGC
TATACCGCAAACAAGAAAGGGGTTGATGGCGTCACTCCCGGTGCTACGCTCTTGGCGAAGTTCGCTCCGCCCCAGTGCCG
CTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-187)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50

100

0.513
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.313

100

0.401
  pilA2 Legionella pneumophila str. Paris

41.011

95.187

0.39
  pilA2 Legionella pneumophila strain ERS1305867

41.011

95.187

0.39