Detailed information NTG0728957

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N4T38_RS04620
• Genome accession: NZ_CP104351
• Coordinates: 908953..910071 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain 2021CK-01335
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 903953..915071

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4T38_RS04590 (N4T38_04590)	-	904782..905630 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
N4T38_RS04595 (N4T38_04595)	-	905883..906338 (+)	456	WP_000782976.1	bacteriohemerythrin	-
N4T38_RS04600 (N4T38_04600)	-	906491..907558 (+)	1068	WP_029642282.1	membrane protein	-
N4T38_RS04605 (N4T38_04605)	-	907558..907881 (+)	324	WP_000442585.1	RnfH family protein	-
N4T38_RS04610 (N4T38_04610)	-	907936..908334 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
N4T38_RS04615 (N4T38_04615)	fur	908446..908883 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
N4T38_RS04620 (N4T38_04620)	pilU	908953..910071 (-)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N4T38_RS04625 (N4T38_04625)	pilT	910099..911136 (-)	1038	WP_000355487.1	type IV pilus twitching motility protein PilT	Machinery gene
N4T38_RS04630 (N4T38_04630)	-	911263..911955 (+)	693	WP_118896106.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=728957 N4T38_RS04620 WP_000347041.1 908953..910071(-) (pilU) [Acinetobacter baumannii strain 2021CK-01335]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=728957 N4T38_RS04620 WP_000347041.1 908953..910071(-) (pilU) [Acinetobacter baumannii strain 2021CK-01335]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392