Detailed information NTG0728894

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N4T37_RS03780
• Genome accession: NZ_CP104350
• Coordinates: 344930..346048 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain 2021CK-01333
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 339930..351048

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4T37_RS03770 (N4T37_03765)	-	343046..343738 (-)	693	WP_118896106.1	YggS family pyridoxal phosphate-dependent enzyme	-
N4T37_RS03775 (N4T37_03770)	pilT	343865..344902 (+)	1038	WP_000355487.1	type IV pilus twitching motility protein PilT	Machinery gene
N4T37_RS03780 (N4T37_03775)	pilU	344930..346048 (+)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N4T37_RS03785 (N4T37_03780)	fur	346118..346555 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
N4T37_RS03790 (N4T37_03785)	-	346667..347065 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
N4T37_RS03795 (N4T37_03790)	-	347122..347445 (-)	324	WP_000442585.1	RnfH family protein	-
N4T37_RS03800 (N4T37_03795)	-	347445..348512 (-)	1068	WP_029642282.1	membrane protein	-
N4T37_RS03805 (N4T37_03800)	-	348665..349120 (-)	456	WP_000782976.1	bacteriohemerythrin	-
N4T37_RS03810 (N4T37_03805)	-	349373..350221 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=728894 N4T37_RS03780 WP_000347041.1 344930..346048(+) (pilU) [Acinetobacter baumannii strain 2021CK-01333]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=728894 N4T37_RS03780 WP_000347041.1 344930..346048(+) (pilU) [Acinetobacter baumannii strain 2021CK-01333]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392