Detailed information NTG0728800

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N4T41_RS20100
• Genome accession: NZ_CP104340
• Coordinates: 3761050..3762168 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain 2021CK-01409
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3756050..3767168

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4T41_RS20070 (N4T41_20080)	-	3756878..3757726 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
N4T41_RS20075 (N4T41_20085)	-	3757979..3758434 (+)	456	WP_000782976.1	bacteriohemerythrin	-
N4T41_RS20080 (N4T41_20090)	-	3758587..3759654 (+)	1068	WP_029642282.1	membrane protein	-
N4T41_RS20085 (N4T41_20095)	-	3759654..3759977 (+)	324	WP_000442585.1	RnfH family protein	-
N4T41_RS20090 (N4T41_20100)	-	3760033..3760431 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
N4T41_RS20095 (N4T41_20105)	fur	3760543..3760980 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
N4T41_RS20100 (N4T41_20110)	pilU	3761050..3762168 (-)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N4T41_RS20105 (N4T41_20115)	pilT	3762196..3763233 (-)	1038	WP_000355487.1	type IV pilus twitching motility protein PilT	Machinery gene
N4T41_RS20110 (N4T41_20120)	-	3763360..3764052 (+)	693	WP_118896106.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=728800 N4T41_RS20100 WP_000347041.1 3761050..3762168(-) (pilU) [Acinetobacter baumannii strain 2021CK-01409]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=728800 N4T41_RS20100 WP_000347041.1 3761050..3762168(-) (pilU) [Acinetobacter baumannii strain 2021CK-01409]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392