Detailed information NTG0728711

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N4T40_RS01805
• Genome accession: NZ_CP104335
• Coordinates: 344934..346052 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain 2021CK-01408
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 339934..351052

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4T40_RS01795 (N4T40_01795)	-	343050..343742 (-)	693	WP_118896106.1	YggS family pyridoxal phosphate-dependent enzyme	-
N4T40_RS01800 (N4T40_01800)	pilT	343869..344906 (+)	1038	WP_000355487.1	type IV pilus twitching motility protein PilT	Machinery gene
N4T40_RS01805 (N4T40_01805)	pilU	344934..346052 (+)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N4T40_RS01810 (N4T40_01810)	fur	346122..346559 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
N4T40_RS01815 (N4T40_01815)	-	346671..347069 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
N4T40_RS01820 (N4T40_01820)	-	347124..347447 (-)	324	WP_000442585.1	RnfH family protein	-
N4T40_RS01825 (N4T40_01825)	-	347447..348514 (-)	1068	WP_029642282.1	membrane protein	-
N4T40_RS01830 (N4T40_01830)	-	348667..349122 (-)	456	WP_000782976.1	bacteriohemerythrin	-
N4T40_RS01835 (N4T40_01835)	-	349375..350223 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=728711 N4T40_RS01805 WP_000347041.1 344934..346052(+) (pilU) [Acinetobacter baumannii strain 2021CK-01408]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=728711 N4T40_RS01805 WP_000347041.1 344934..346052(+) (pilU) [Acinetobacter baumannii strain 2021CK-01408]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392