Detailed information NTG0728567

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: N4J17_RS02450
• Genome accession: NZ_CP104311
• Coordinates: 532627..533691 (-)
• Length: 354 a.a.
• NCBI ID: WP_198322318.1
• Uniprot ID: -
• Organism: Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 527627..538691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4J17_RS02425 (N4J17_02425)	aroK	528074..528613 (-)	540	WP_198322313.1	shikimate kinase AroK	-
N4J17_RS02430 (N4J17_02430)	pilQ	528675..530864 (-)	2190	WP_198322314.1	type IV pilus secretin PilQ	-
N4J17_RS02435 (N4J17_02435)	-	530851..531393 (-)	543	WP_198322315.1	pilus assembly protein PilP	-
N4J17_RS02440 (N4J17_02440)	-	531393..532034 (-)	642	WP_198322316.1	type 4a pilus biogenesis protein PilO	-
N4J17_RS02445 (N4J17_02445)	-	532031..532630 (-)	600	WP_198322317.1	PilN domain-containing protein	-
N4J17_RS02450 (N4J17_02450)	pilM	532627..533691 (-)	1065	WP_198322318.1	pilus assembly protein PilM	Machinery gene
N4J17_RS02455 (N4J17_02455)	-	534018..536465 (+)	2448	WP_277458150.1	penicillin-binding protein 1A	-
N4J17_RS02460 (N4J17_02460)	-	536481..537092 (+)	612	WP_277458151.1	hypothetical protein	-
N4J17_RS02465 (N4J17_02465)	rpmE	537134..537337 (-)	204	WP_198322320.1	50S ribosomal protein L31	-
N4J17_RS02470 (N4J17_02470)	-	537440..538213 (+)	774	WP_198322321.1	exodeoxyribonuclease III	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38708.16 Da
• Isoelectric Point: 4.9453

>NTDB_id=728567 N4J17_RS02450 WP_198322318.1 532627..533691(-) (pilM) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
MWGFGRKKPPLLGIDISAAAVKLLELSRKNGGYQVESYGVVPLPRNTVVDNTIAEPDNVSAAVRAVVKQSGTPLRHAVVA
VPGSVVITKRFTIPANLEGDQLEAQIELEADQYIPHAREEVSLDFEVLGKSTKNPDLVDVLLVATRRENVEDRVSVLENA
GLGVEIVDVESYGIERAFELVKDQLPPLLRDRAVAVADVGAVATTLNVIHDGSIIYTREQGFGGMQLTDEIQRRFGLTYE
EAGLAKKEGSLPGNYAEEVLEPFKHALVQQIRRSFQFYLSSTAQRGFDAVVLAGGCAMIPEIDRYVEAALQVPTVVANPF
RSMSFSGRVRQERLNYDASALMLACGLALRSFDR

Nucleotide

• Length: 1065 bp

>NTDB_id=728567 N4J17_RS02450 WP_198322318.1 532627..533691(-) (pilM) [Methylococcus capsulatus strain VKPM-B-12549 (GBS-15)]
ATGTGGGGTTTCGGAAGGAAGAAACCGCCTCTGCTGGGGATCGACATCAGCGCGGCGGCGGTCAAACTGCTCGAACTGAG
CAGAAAGAACGGCGGCTACCAGGTGGAAAGCTACGGCGTCGTGCCTTTGCCCCGCAACACGGTGGTCGATAATACCATTG
CCGAGCCGGACAATGTGTCGGCCGCGGTACGGGCGGTGGTCAAACAGTCGGGAACCCCGCTCAGGCACGCGGTGGTGGCA
GTGCCTGGCTCAGTGGTCATCACTAAGCGCTTCACCATCCCCGCTAACCTGGAGGGTGACCAACTCGAGGCGCAGATCGA
ACTGGAGGCGGACCAGTACATCCCTCATGCCAGGGAGGAGGTCAGTCTGGATTTTGAGGTGCTGGGGAAGAGCACGAAAA
ACCCCGATCTGGTGGATGTGCTTTTGGTGGCGACCCGCCGGGAGAACGTGGAGGATCGGGTCTCGGTTCTGGAAAACGCA
GGGCTCGGCGTCGAAATCGTCGATGTCGAATCCTATGGCATCGAACGTGCCTTCGAGCTGGTCAAGGACCAGCTTCCGCC
GCTCCTCCGCGACCGGGCGGTAGCCGTGGCCGACGTGGGGGCCGTGGCCACCACGCTCAACGTCATCCACGACGGTTCGA
TCATCTATACCCGGGAACAGGGTTTCGGTGGCATGCAGCTCACCGACGAGATCCAGCGGCGCTTCGGTCTGACCTACGAG
GAAGCGGGGCTGGCGAAGAAAGAGGGGAGCCTTCCCGGCAACTACGCAGAGGAAGTGCTGGAGCCGTTCAAGCACGCTCT
GGTTCAGCAGATCCGCCGGTCGTTCCAGTTCTATCTCTCGTCCACTGCTCAGCGTGGCTTCGATGCGGTGGTGCTGGCCG
GCGGCTGCGCCATGATCCCGGAAATCGACCGTTACGTCGAGGCGGCATTGCAGGTTCCCACCGTCGTCGCCAATCCTTTC
CGGAGCATGAGTTTTTCAGGGCGGGTGCGGCAGGAGCGGCTGAATTACGACGCTTCGGCCCTGATGCTGGCCTGCGGCCT
GGCGCTGCGGAGTTTCGACCGATGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	50.729	96.893	0.492
comM	Acinetobacter nosocomialis M2	50.146	96.893	0.486
comM	Acinetobacter baylyi ADP1	48	98.87	0.475
pilM	Legionella pneumophila strain ERS1305867	42.241	98.305	0.415