Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   W01_RS08815 Genome accession   NZ_AP019547
Coordinates   1974826..1976184 (+) Length   452 a.a.
NCBI ID   WP_173053915.1    Uniprot ID   A0A455X4I5
Organism   Candidatus Nitrotoga sp. AM1P     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1974826..1988161 1974826..1976184 within 0


Gene organization within MGE regions


Location: 1974826..1988161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  W01_RS08815 (W01_17250) pilR 1974826..1976184 (+) 1359 WP_173053915.1 sigma-54 dependent transcriptional regulator Regulator
  W01_RS08820 (W01_17260) ampD 1976188..1976751 (+) 564 WP_173053917.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  W01_RS08825 (W01_17270) - 1977046..1979802 (+) 2757 WP_173053919.1 ribonucleoside-diphosphate reductase subunit alpha -
  W01_RS08830 (W01_17280) - 1979799..1980848 (+) 1050 WP_242006921.1 ribonucleotide-diphosphate reductase subunit beta -
  W01_RS08835 - 1980862..1981344 (-) 483 WP_173053923.1 hypothetical protein -
  W01_RS08840 - 1981323..1981607 (-) 285 WP_173053925.1 hypothetical protein -
  W01_RS08845 (W01_17300) msbA 1981951..1983708 (+) 1758 WP_173053927.1 lipid A export permease/ATP-binding protein MsbA -
  W01_RS08850 (W01_17310) - 1983854..1984864 (-) 1011 WP_173053929.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  W01_RS08855 (W01_17320) - 1984920..1985801 (+) 882 WP_173053932.1 SDR family oxidoreductase -
  W01_RS08860 (W01_17330) - 1985826..1986425 (-) 600 WP_173053934.1 TIGR02466 family protein -
  W01_RS08865 (W01_17340) - 1986500..1986814 (-) 315 WP_173053936.1 DUF1840 domain-containing protein -
  W01_RS08870 (W01_17350) - 1987316..1988161 (+) 846 WP_173053938.1 gamma-glutamylcyclotransferase family protein -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49907.60 Da        Isoelectric Point: 5.2572

>NTDB_id=72849 W01_RS08815 WP_173053915.1 1974826..1976184(+) (pilR) [Candidatus Nitrotoga sp. AM1P]
MTVDIQASPARVLVVDDEPDILELIELALLRMGLQVDRASNVQEALQQLDTKTYQLCLTDMRLPDGEGLEVVQYIIARNM
HVPVAVITAHGNMENAIFALKAGAFDYLPKPVALTQLRALVNAVLKLPPLSATVRKGVRTLLGLSPAMEKVRDLIDRVAR
SQAPVHISGESGSGKELAARLIVEKSARRDQPFITVNCGAIPETLMESEFFGYRKGAFTGAEKDREGLFQAANGGTLFLD
EVADLPLSMQVKLLRVIQEKKVRKVGATQEESVDVRIISATHNALAEQVQQGKFRQDLYYRLNVITLLMPPLRAMRDDIA
EVANHFLARLCGDAKVELASSALKALQQYDFPGNVRELENIIERALALCSDDIIMPADLQLAPTDFAETTTKPSVAGKYP
LTDYLDSVERAAILEALQQTNFNRTAAAKVLGVTFRALRYRMDRLDITDKDE

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=72849 W01_RS08815 WP_173053915.1 1974826..1976184(+) (pilR) [Candidatus Nitrotoga sp. AM1P]
ATGACCGTTGATATACAGGCCAGCCCTGCGCGCGTCTTGGTGGTGGATGACGAGCCGGATATATTGGAGTTGATCGAGTT
GGCGCTGCTACGCATGGGATTGCAGGTCGACCGCGCTAGTAATGTGCAAGAAGCTTTGCAACAATTAGATACTAAAACAT
ATCAGCTATGTCTGACAGATATGCGCCTGCCGGATGGAGAAGGACTGGAGGTGGTGCAATATATTATTGCTCGCAACATG
CATGTGCCAGTCGCGGTAATTACCGCGCATGGCAACATGGAAAATGCGATTTTTGCACTTAAGGCGGGCGCTTTCGATTA
CCTCCCCAAGCCAGTTGCGCTGACCCAGCTGCGCGCCTTGGTCAATGCTGTGCTAAAGCTGCCGCCACTGTCTGCCACCG
TACGAAAAGGCGTGCGAACATTACTGGGACTTTCCCCGGCCATGGAAAAGGTGCGCGACCTGATCGATCGGGTGGCACGT
AGCCAGGCACCGGTACATATCAGTGGCGAATCAGGTAGTGGAAAAGAACTGGCTGCGCGGCTGATCGTTGAAAAAAGCGC
TCGACGCGACCAGCCTTTCATCACGGTAAACTGCGGTGCAATCCCGGAAACGCTTATGGAAAGCGAATTTTTCGGTTATC
GCAAGGGCGCCTTCACTGGTGCGGAAAAAGATCGCGAGGGTTTATTTCAGGCTGCCAATGGCGGTACTTTATTCCTTGAC
GAGGTTGCCGATTTACCTCTCTCCATGCAGGTAAAATTGTTGCGGGTAATCCAGGAGAAAAAAGTGCGTAAGGTGGGCGC
CACGCAAGAAGAGTCGGTGGATGTACGTATCATCAGCGCCACCCATAATGCGTTGGCGGAGCAAGTACAGCAGGGGAAAT
TCCGTCAGGATTTATATTACCGTCTGAATGTGATTACCCTGCTTATGCCACCGTTGCGCGCAATGCGCGATGACATTGCC
GAGGTCGCCAACCATTTTTTAGCTCGTTTGTGCGGTGATGCAAAGGTCGAATTAGCATCGTCAGCTTTGAAAGCACTTCA
ACAATACGATTTTCCGGGCAACGTGCGCGAATTGGAAAATATAATCGAACGGGCACTTGCATTATGTTCGGACGACATTA
TCATGCCAGCCGATTTGCAGCTTGCACCCACGGATTTTGCTGAAACGACCACCAAACCAAGTGTAGCTGGCAAATATCCA
CTTACCGATTATCTGGATAGCGTCGAACGCGCAGCCATTCTAGAGGCGTTACAGCAAACCAATTTCAACCGCACAGCTGC
GGCCAAGGTGCTGGGAGTGACTTTTCGCGCTTTGCGCTATCGTATGGATCGATTGGATATCACTGACAAGGATGAATAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(12-121)

Sigma-54 interaction domain (Sigma54_activat)

(142-307)

Bacterial regulatory protein, Fis family (HTH_8)

(405-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A455X4I5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

57.336

98.009

0.562
  pilR Acinetobacter baumannii strain A118

45.788

100

0.469
  luxO Vibrio cholerae strain A1552

37.986

96.681

0.367

Multiple sequence alignment