Detailed information NTG0728389

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N4Q44_RS14480
• Genome accession: NZ_CP104295
• Coordinates: 3074983..3076101 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain DETAB-C9
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3069983..3081101

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N4Q44_RS14470 (N4Q44_14480)	-	3073099..3073791 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
N4Q44_RS14475 (N4Q44_14485)	pilT	3073918..3074955 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
N4Q44_RS14480 (N4Q44_14490)	pilU	3074983..3076101 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N4Q44_RS14485 (N4Q44_14495)	fur	3076171..3076608 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
N4Q44_RS14490 (N4Q44_14500)	bamE	3076720..3077118 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
N4Q44_RS14495 (N4Q44_14505)	-	3077173..3077496 (-)	324	WP_000442585.1	RnfH family protein	-
N4Q44_RS14500 (N4Q44_14510)	-	3077496..3078563 (-)	1068	WP_004748097.1	hypothetical protein	-
N4Q44_RS14505 (N4Q44_14515)	-	3078716..3079171 (-)	456	WP_000782976.1	bacteriohemerythrin	-
N4Q44_RS14510 (N4Q44_14520)	-	3079424..3080272 (-)	849	WP_071210971.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=728389 N4Q44_RS14480 WP_000347034.1 3074983..3076101(+) (pilU) [Acinetobacter baumannii strain DETAB-C9]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=728389 N4Q44_RS14480 WP_000347034.1 3074983..3076101(+) (pilU) [Acinetobacter baumannii strain DETAB-C9]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAATAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39