Detailed information NTG0727136

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MRX62_RS12665
• Genome accession: NZ_CP104027
• Coordinates: 2656600..2657730 (-)
• Length: 376 a.a.
• NCBI ID: WP_010894128.1
• Uniprot ID: Q9PCX2
• Organism: Xylella fastidiosa subsp. pauca strain 50088
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2651600..2662730

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MRX62_RS12645 (MRX62_12660)	-	2651671..2652429 (+)	759	WP_010894122.1	LytTR family DNA-binding domain-containing protein	-
MRX62_RS12650 (MRX62_12665)	hemC	2652446..2653363 (+)	918	WP_010894123.1	hydroxymethylbilane synthase	-
MRX62_RS12655 (MRX62_12670)	-	2653417..2654226 (-)	810	WP_031336664.1	YdiY family protein	-
MRX62_RS12660 (MRX62_12675)	-	2655070..2656083 (+)	1014	WP_229716473.1	YafY family protein	-
MRX62_RS12665 (MRX62_12680)	pilU	2656600..2657730 (-)	1131	WP_010894128.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MRX62_RS12670 (MRX62_12685)	pilT	2657896..2658930 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
MRX62_RS12675 (MRX62_12690)	-	2658999..2659196 (-)	198	WP_010894129.1	hypothetical protein	-
MRX62_RS12680 (MRX62_12695)	-	2660193..2661020 (+)	828	WP_173405994.1	glycosyltransferase family 2 protein	-
MRX62_RS12685 (MRX62_12700)	-	2661684..2662508 (-)	825	WP_010894133.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42476.67 Da
• Isoelectric Point: 6.5027

>NTDB_id=727136 MRX62_RS12665 WP_010894128.1 2656600..2657730(-) (pilU) [Xylella fastidiosa subsp. pauca strain 50088]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITN
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLTDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=727136 MRX62_RS12665 WP_010894128.1 2656600..2657730(-) (pilU) [Xylella fastidiosa subsp. pauca strain 50088]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAAGTCCATGAATGCAACTTCGCAATTGACATCACCAAC
ATTGGTCGCTTCCGCGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACTCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCAGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGACTGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACTACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCCAAAACTCTGAGTCAAGGATTCGATGGAGTAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-272)

3D structure

• AlphaFold DB: Q9PCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	59.887	94.149	0.564
pilU	Vibrio cholerae strain A1552	51.124	94.681	0.484
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	96.809	0.399