Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NYP54_RS08430 Genome accession   NZ_CP103983
Coordinates   1939995..1941701 (-) Length   568 a.a.
NCBI ID   WP_117003993.1    Uniprot ID   -
Organism   Cysteiniphilum sp. QT6929     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1934995..1946701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYP54_RS08410 (NYP54_08410) - 1936456..1936698 (-) 243 WP_283156131.1 hypothetical protein -
  NYP54_RS08415 (NYP54_08415) - 1936767..1937639 (-) 873 WP_283155809.1 IS982 family transposase -
  NYP54_RS08420 (NYP54_08420) - 1937838..1938758 (+) 921 WP_283156132.1 hypothetical protein -
  NYP54_RS08425 (NYP54_08425) - 1938755..1939975 (-) 1221 WP_283156133.1 type II secretion system F family protein -
  NYP54_RS08430 (NYP54_08430) pilB 1939995..1941701 (-) 1707 WP_117003993.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NYP54_RS08435 (NYP54_08435) bamE 1941779..1942126 (-) 348 WP_151194311.1 outer membrane protein assembly factor BamE -
  NYP54_RS08440 (NYP54_08440) - 1942162..1942749 (-) 588 WP_283156134.1 SIS domain-containing protein -
  NYP54_RS08445 (NYP54_08445) - 1942848..1943612 (+) 765 WP_117003996.1 hypothetical protein -
  NYP54_RS08450 (NYP54_08450) - 1943635..1944771 (-) 1137 WP_283156135.1 ABC transporter permease -
  NYP54_RS08455 (NYP54_08455) - 1944768..1945718 (-) 951 WP_283156136.1 ABC transporter ATP-binding protein -
  NYP54_RS08460 (NYP54_08460) - 1945734..1946687 (-) 954 WP_283156137.1 HlyD family efflux transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63854.08 Da        Isoelectric Point: 5.1210

>NTDB_id=726772 NYP54_RS08430 WP_117003993.1 1939995..1941701(-) (pilB) [Cysteiniphilum sp. QT6929]
MTQHGISSFLLSQNLINNDDLEQAIDEIDNSNEPLITHLINKKLLDANAFAIESSQFFNLPLFDLNAYDLDFFPRAFLDE
RLINDYVCLPLYKKDNTLLLATSDPLNLNAVREFRFFSNLDIEQIIVDDTHLRRFIKEVTENTTDNAMKTLEGMDLEEID
LDIVEADDFEDDDDFGDNDEPVIKYINKIIVDAINKGASDIHFEPYATNFRIRYRIDGLLQEIATPPLKIAARTAARIKI
MSQLNIAEKRIPQDGRFKLKISKTHVIDFRVSTCPTAFGEKVVLRILDQAKTNLSVDKLGFEPHQQEIYLAALKQSQGMI
LVTGPTGSGKTVTLYTGLHIINTIDKNISTAEDPVEINVNGINQVQINNKQGLTFARALKSFLRQDPDIIMIGEIRDFET
ADIAIKAAQTGHLVLSTVHTNSAPETLNRLLDMGIPHYNIISSVSLIIAQRLARKLCPHCKKPAPDISRELLLDMGIRPV
HVEKEFTAYTNNPQGCARCFKGFRGRVAIYEVMPLSLAMSRLIVEGRSTLTLAEQAQREGIDNLQQSALIKVVHGVTSIE
EAHRVTSM

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=726772 NYP54_RS08430 WP_117003993.1 1939995..1941701(-) (pilB) [Cysteiniphilum sp. QT6929]
ATGACTCAGCACGGAATCAGTAGTTTTCTACTTAGCCAAAACCTTATTAATAATGATGATCTTGAACAAGCTATTGATGA
AATAGACAATAGCAATGAGCCGCTTATCACTCATTTGATCAACAAGAAGCTACTTGATGCCAATGCATTTGCCATTGAAT
CATCGCAGTTTTTTAACTTGCCTCTTTTTGATCTGAATGCTTATGATTTGGATTTTTTCCCACGTGCTTTTTTGGATGAG
CGTTTAATTAATGACTACGTCTGTTTGCCACTTTACAAAAAAGACAACACATTACTATTAGCAACCAGTGATCCATTAAA
CCTAAACGCCGTACGTGAGTTTCGCTTTTTCAGCAATCTAGATATTGAACAAATCATTGTTGATGACACACACCTTAGAC
GCTTCATCAAAGAAGTGACCGAGAACACCACTGACAATGCTATGAAAACGCTTGAAGGCATGGATCTTGAAGAAATTGAC
CTTGATATTGTTGAGGCTGATGACTTTGAAGATGATGATGACTTTGGTGATAACGATGAGCCGGTGATCAAATACATCAA
TAAGATTATTGTTGATGCAATTAATAAAGGCGCTTCGGATATCCACTTTGAACCGTATGCTACAAACTTCAGAATTCGTT
ATCGGATTGATGGACTTTTGCAAGAGATTGCCACCCCCCCACTTAAAATCGCCGCACGAACTGCCGCTAGAATCAAAATC
ATGTCTCAACTAAATATTGCAGAGAAACGTATTCCGCAAGATGGACGTTTTAAATTAAAAATCTCCAAAACACATGTCAT
TGATTTTAGGGTCAGCACCTGTCCAACGGCATTTGGTGAGAAAGTTGTTTTAAGGATTTTGGATCAGGCAAAGACTAATC
TTAGTGTTGATAAACTGGGCTTTGAACCGCACCAACAAGAAATTTATCTCGCGGCATTAAAACAATCTCAAGGCATGATC
TTGGTAACAGGTCCAACAGGAAGTGGTAAGACAGTCACACTTTATACTGGACTACACATTATCAACACCATTGATAAGAA
TATCTCAACCGCCGAAGACCCAGTTGAAATCAATGTTAATGGTATTAATCAGGTGCAAATCAATAACAAGCAGGGTCTGA
CCTTTGCCCGAGCCTTGAAGTCTTTCTTACGTCAAGATCCCGATATTATTATGATTGGTGAGATTCGTGACTTTGAAACA
GCAGATATTGCCATTAAAGCCGCACAAACGGGGCATTTGGTATTATCTACTGTACATACCAACAGCGCACCGGAAACCTT
AAACCGCCTATTAGACATGGGAATTCCGCACTATAATATTATCTCCTCTGTGTCACTGATTATCGCGCAGCGCTTGGCAC
GTAAGCTCTGCCCACATTGTAAAAAACCAGCTCCTGATATTAGCCGTGAATTATTATTAGACATGGGTATTCGCCCAGTT
CATGTTGAAAAAGAGTTTACCGCATATACCAATAACCCACAGGGTTGCGCGCGTTGTTTTAAAGGATTTCGTGGACGCGT
TGCTATTTATGAAGTGATGCCATTGAGTCTTGCGATGAGTCGCTTGATTGTCGAGGGTCGCAGCACACTAACGCTTGCTG
AACAGGCACAGCGCGAAGGGATTGATAATCTACAGCAGTCTGCTTTAATCAAAGTAGTGCATGGTGTCACTTCAATTGAA
GAAGCGCACCGCGTTACCTCAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

50.088

100

0.502
  pilB Acinetobacter baumannii D1279779

49.385

100

0.495
  pilB Legionella pneumophila strain ERS1305867

51.103

95.775

0.489
  pilB Vibrio cholerae strain A1552

46.113

99.648

0.46
  pilB Vibrio campbellii strain DS40M4

45.73

98.944

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.811

98.768

0.452
  pilF Neisseria gonorrhoeae MS11

43.47

98.415

0.428