Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   M2494_RS16605 Genome accession   NZ_AP019411
Coordinates   3743587..3744156 (-) Length   189 a.a.
NCBI ID   WP_003426596.1    Uniprot ID   A0A0K8M6E3
Organism   Pseudomonas syringae pv. actinidiae strain MAFF613020     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3716500..3743444 3743587..3744156 flank 143


Gene organization within MGE regions


Location: 3716500..3744156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2494_RS16470 (KPSA1B_103364) - 3716500..3716922 (-) 423 WP_003379169.1 SMI1/KNR4 family protein -
  M2494_RS29940 (KPSA1B_103365) - 3717002..3717253 (+) 252 WP_003379170.1 hypothetical protein -
  M2494_RS16475 (KPSA1B_103366) - 3717363..3717788 (-) 426 WP_003379171.1 hypothetical protein -
  M2494_RS16480 (KPSA1B_103367) - 3718139..3718702 (+) 564 WP_019334593.1 hypothetical protein -
  M2494_RS16485 (KPSA1B_103368) - 3718716..3719732 (-) 1017 WP_003373643.1 IS30-like element ISPsy38 family transposase -
  M2494_RS16490 (KPSA1B_103369) - 3719976..3720194 (+) 219 WP_003379173.1 hypothetical protein -
  M2494_RS16495 (KPSA1B_103370) - 3720741..3721808 (-) 1068 WP_077142725.1 IS630-like element ISPsy32 family transposase -
  M2494_RS16500 (KPSA1B_103371) - 3721998..3722717 (+) 720 WP_017699035.1 hypothetical protein -
  M2494_RS16505 (KPSA1B_103372) - 3722893..3723663 (+) 771 WP_003379175.1 SDR family NAD(P)-dependent oxidoreductase -
  M2494_RS16510 (KPSA1B_103373) - 3723811..3724062 (-) 252 WP_003379176.1 hypothetical protein -
  M2494_RS16515 (KPSA1B_103374) - 3724277..3724798 (+) 522 WP_003379177.1 hypothetical protein -
  M2494_RS16520 (KPSA1B_103375) - 3724826..3725956 (-) 1131 WP_003379178.1 ROK family protein -
  M2494_RS16525 (KPSA1B_103376) - 3726122..3727573 (+) 1452 WP_003379179.1 extracellular solute-binding protein -
  M2494_RS16530 (KPSA1B_103377) - 3727570..3728463 (+) 894 WP_017683899.1 sugar ABC transporter permease -
  M2494_RS16535 (KPSA1B_103378) - 3728460..3729353 (+) 894 WP_003379184.1 ABC transporter permease subunit -
  M2494_RS16540 (KPSA1B_103379) ugpC 3729350..3730456 (+) 1107 WP_003379187.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  M2494_RS16545 (KPSA1B_103380) - 3730463..3731224 (+) 762 WP_003379189.1 glycerophosphoryl diester phosphodiesterase -
  M2494_RS16550 (KPSA1B_103381) - 3731221..3731964 (+) 744 WP_003379192.1 inositol monophosphatase family protein -
  M2494_RS16555 (KPSA1B_103382) - 3732087..3732386 (+) 300 WP_003379193.1 hypothetical protein -
  M2494_RS16560 (KPSA1B_103383) - 3733281..3734348 (+) 1068 WP_077142726.1 IS630-like element ISPsy32 family transposase -
  M2494_RS16565 (KPSA1B_103385) - 3734584..3735651 (+) 1068 WP_077142725.1 IS630-like element ISPsy32 family transposase -
  M2494_RS16570 - 3735658..3736110 (-) 453 WP_019334430.1 hypothetical protein -
  M2494_RS16575 (KPSA1B_103386) tnpB 3736171..3736530 (+) 360 WP_005621010.1 IS66 family insertion sequence element accessory protein TnpB -
  M2494_RS16580 (KPSA1B_103387) - 3736548..3738080 (+) 1533 WP_077142906.1 IS66 family transposase -
  M2494_RS16585 (KPSA1B_103389) - 3738296..3738709 (-) 414 WP_003381885.1 hypothetical protein -
  M2494_RS16590 (KPSA1B_103390) - 3738702..3740711 (-) 2010 WP_017699151.1 hypothetical protein -
  M2494_RS16595 (KPSA1B_103391) - 3740704..3742230 (-) 1527 WP_004397020.1 site-specific integrase -
  M2494_RS16600 (KPSA1B_103392) - 3742227..3743444 (-) 1218 WP_004397021.1 site-specific integrase -
  M2494_RS16605 (KPSA1B_103393) ssb 3743587..3744156 (-) 570 WP_003426596.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 189 a.a.        Molecular weight: 20974.10 Da        Isoelectric Point: 5.9404

>NTDB_id=72624 M2494_RS16605 WP_003426596.1 3743587..3744156(-) (ssb) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
MARGVNKVILVGTCGQDPEVRYLPNGNAVTNLSLATSEQWTDKQSGQKVEKTEWHRVSMFGKVAEIAGEYLRKGSQVYIE
GKLQTREWEKDGIKRYTTEIVVDMQGTMQLLGGRPQGDAQQGQGGGGNYNQSAPRPQQSRPQQSAPQQSAPQQNYNQQPP
QQRDSRPAPQQQAPQPAADFDSFDDDIPF

Nucleotide


Download         Length: 570 bp        

>NTDB_id=72624 M2494_RS16605 WP_003426596.1 3743587..3744156(-) (ssb) [Pseudomonas syringae pv. actinidiae strain MAFF613020]
ATGGCCCGTGGGGTTAACAAAGTCATATTGGTCGGTACATGCGGCCAGGATCCCGAAGTTCGCTACTTGCCTAACGGTAA
CGCCGTGACCAACCTGAGTCTGGCAACCAGCGAACAATGGACTGACAAGCAGAGCGGCCAGAAGGTCGAGAAGACCGAGT
GGCACCGTGTTTCGATGTTCGGCAAGGTTGCCGAAATCGCCGGCGAATACCTGCGCAAGGGTTCGCAGGTCTACATCGAA
GGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACTGAAATCGTCGTCGACATGCAAGGCAC
CATGCAACTGCTGGGCGGCCGTCCACAGGGCGACGCTCAACAAGGTCAGGGCGGTGGCGGCAACTACAACCAGTCTGCAC
CTCGTCCACAGCAGTCGCGCCCACAGCAATCGGCGCCGCAGCAATCTGCCCCTCAGCAGAACTACAACCAGCAGCCGCCA
CAACAGCGCGATTCGCGCCCAGCGCCGCAACAACAAGCGCCGCAGCCTGCTGCTGACTTTGACAGCTTTGATGACGATAT
TCCGTTCTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(5-107)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K8M6E3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

55.208

100

0.561
  ssb Glaesserella parasuis strain SC1401

47.368

100

0.476
  ssb Neisseria meningitidis MC58

44.681

99.471

0.444
  ssb Neisseria gonorrhoeae MS11

44.681

99.471

0.444

Multiple sequence alignment