Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NUE47_RS07320 Genome accession   NZ_CP103864
Coordinates   1479877..1481001 (-) Length   374 a.a.
NCBI ID   WP_000228544.1    Uniprot ID   A0A9P2PX49
Organism   Escherichia coli O157:H7 strain RV06     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1474877..1486001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUE47_RS07290 (NUE47_07290) yrdA 1476677..1477231 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  NUE47_RS07295 (NUE47_07295) yrdB 1477207..1477464 (-) 258 WP_001070590.1 DUF1488 domain-containing protein -
  NUE47_RS07300 (NUE47_07300) aroE 1477461..1478279 (-) 819 WP_000451254.1 shikimate dehydrogenase -
  NUE47_RS07305 (NUE47_07305) tsaC 1478284..1478856 (-) 573 WP_001301437.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  NUE47_RS07310 (NUE47_07310) yrdD 1478861..1479403 (-) 543 WP_001129722.1 type I DNA topoisomerase -
  NUE47_RS07315 (NUE47_07315) smg 1479432..1479905 (-) 474 WP_000460672.1 DUF494 family protein Smg -
  NUE47_RS07320 (NUE47_07320) dprA 1479877..1481001 (-) 1125 WP_000228544.1 DNA-protecting protein DprA Machinery gene
  NUE47_RS07325 (NUE47_07325) def 1481131..1481640 (+) 510 WP_000114984.1 peptide deformylase -
  NUE47_RS07330 (NUE47_07330) fmt 1481655..1482602 (+) 948 WP_000004459.1 methionyl-tRNA formyltransferase -
  NUE47_RS07335 (NUE47_07335) rsmB 1482648..1483937 (+) 1290 WP_001301795.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  NUE47_RS07340 (NUE47_07340) trkA 1483959..1485335 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  NUE47_RS07345 (NUE47_07345) mscL 1485465..1485875 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41005.96 Da        Isoelectric Point: 6.2655

>NTDB_id=726230 NUE47_RS07320 WP_000228544.1 1479877..1481001(-) (dprA) [Escherichia coli O157:H7 strain RV06]
MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=726230 NUE47_RS07320 WP_000228544.1 1479877..1481001(-) (dprA) [Escherichia coli O157:H7 strain RV06]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Glaesserella parasuis strain SC1401

45.401

90.107

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393