Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NX081_RS13190 Genome accession   NZ_CP103856
Coordinates   2664603..2665673 (-) Length   356 a.a.
NCBI ID   WP_007408320.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM116265     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659603..2670673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX081_RS13140 (NX081_13140) sipW 2659663..2660247 (-) 585 WP_060562614.1 signal peptidase I SipW -
  NX081_RS13145 (NX081_13145) tapA 2660219..2660890 (-) 672 WP_060562615.1 amyloid fiber anchoring/assembly protein TapA -
  NX081_RS13150 (NX081_13150) - 2661149..2661478 (+) 330 WP_060562616.1 DUF3889 domain-containing protein -
  NX081_RS13155 (NX081_13155) - 2661518..2661697 (-) 180 WP_003153093.1 YqzE family protein -
  NX081_RS13160 (NX081_13160) comGG 2661754..2662131 (-) 378 WP_060562617.1 competence type IV pilus minor pilin ComGG Machinery gene
  NX081_RS13165 (NX081_13165) comGF 2662132..2662632 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  NX081_RS13170 (NX081_13170) comGE 2662541..2662855 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  NX081_RS13175 (NX081_13175) comGD 2662839..2663276 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  NX081_RS13180 (NX081_13180) comGC 2663266..2663532 (-) 267 WP_060562757.1 competence type IV pilus major pilin ComGC Machinery gene
  NX081_RS13185 (NX081_13185) comGB 2663579..2664616 (-) 1038 WP_060562618.1 competence type IV pilus assembly protein ComGB Machinery gene
  NX081_RS13190 (NX081_13190) comGA 2664603..2665673 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  NX081_RS13195 (NX081_13195) - 2665866..2666816 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  NX081_RS13200 (NX081_13200) - 2666962..2668263 (+) 1302 WP_060562619.1 hemolysin family protein -
  NX081_RS13205 (NX081_13205) - 2668301..2669131 (-) 831 WP_015240214.1 STAS domain-containing protein -
  NX081_RS13215 (NX081_13215) - 2669338..2669718 (-) 381 WP_060562620.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NX081_RS13220 (NX081_13220) - 2669956..2670201 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.79 Da        Isoelectric Point: 9.1996

>NTDB_id=726096 NX081_RS13190 WP_007408320.1 2664603..2665673(-) (comGA) [Bacillus velezensis strain SRCM116265]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=726096 NX081_RS13190 WP_007408320.1 2664603..2665673(-) (comGA) [Bacillus velezensis strain SRCM116265]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTTCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGTTTTCAGGAAGCAAAGGGAGAG
CACGCGAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374