Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NX081_RS13185 Genome accession   NZ_CP103856
Coordinates   2663579..2664616 (-) Length   345 a.a.
NCBI ID   WP_060562618.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM116265     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2658579..2669616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX081_RS13135 (NX081_13135) tasA 2658814..2659599 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  NX081_RS13140 (NX081_13140) sipW 2659663..2660247 (-) 585 WP_060562614.1 signal peptidase I SipW -
  NX081_RS13145 (NX081_13145) tapA 2660219..2660890 (-) 672 WP_060562615.1 amyloid fiber anchoring/assembly protein TapA -
  NX081_RS13150 (NX081_13150) - 2661149..2661478 (+) 330 WP_060562616.1 DUF3889 domain-containing protein -
  NX081_RS13155 (NX081_13155) - 2661518..2661697 (-) 180 WP_003153093.1 YqzE family protein -
  NX081_RS13160 (NX081_13160) comGG 2661754..2662131 (-) 378 WP_060562617.1 competence type IV pilus minor pilin ComGG Machinery gene
  NX081_RS13165 (NX081_13165) comGF 2662132..2662632 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  NX081_RS13170 (NX081_13170) comGE 2662541..2662855 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  NX081_RS13175 (NX081_13175) comGD 2662839..2663276 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  NX081_RS13180 (NX081_13180) comGC 2663266..2663532 (-) 267 WP_060562757.1 competence type IV pilus major pilin ComGC Machinery gene
  NX081_RS13185 (NX081_13185) comGB 2663579..2664616 (-) 1038 WP_060562618.1 competence type IV pilus assembly protein ComGB Machinery gene
  NX081_RS13190 (NX081_13190) comGA 2664603..2665673 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  NX081_RS13195 (NX081_13195) - 2665866..2666816 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  NX081_RS13200 (NX081_13200) - 2666962..2668263 (+) 1302 WP_060562619.1 hemolysin family protein -
  NX081_RS13205 (NX081_13205) - 2668301..2669131 (-) 831 WP_015240214.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39586.84 Da        Isoelectric Point: 9.5987

>NTDB_id=726095 NX081_RS13185 WP_060562618.1 2663579..2664616(-) (comGB) [Bacillus velezensis strain SRCM116265]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLQLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGDLLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGRLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=726095 NX081_RS13185 WP_060562618.1 2663579..2664616(-) (comGB) [Bacillus velezensis strain SRCM116265]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGCAACTACAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCCGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGACTTGTTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACTTCCGGTGCGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTATGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTATCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCGGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.944

93.623

0.617