Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR86_RS05785 Genome accession   NZ_CP103830
Coordinates   1309683..1310981 (-) Length   432 a.a.
NCBI ID   WP_279622985.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 2499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1311920..1325443 1309683..1310981 flank 939


Gene organization within MGE regions


Location: 1309683..1325443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR86_RS05785 (NYR86_05785) comE 1309683..1310981 (-) 1299 WP_279622985.1 type IV pilus secretin PilQ Machinery gene
  NYR86_RS05790 (NYR86_05790) - 1310993..1311397 (-) 405 WP_279622986.1 hypothetical protein -
  NYR86_RS05795 (NYR86_05795) - 1311390..1311917 (-) 528 WP_279438019.1 chromosome segregation protein -
  NYR86_RS05800 (NYR86_05800) - 1311920..1312441 (-) 522 WP_279439257.1 hypothetical protein -
  NYR86_RS05805 (NYR86_05805) - 1312423..1313109 (-) 687 WP_039197447.1 hypothetical protein -
  NYR86_RS05810 (NYR86_05810) - 1313248..1315833 (+) 2586 WP_279622987.1 penicillin-binding protein 1A -
  NYR86_RS05815 (NYR86_05815) aroK 1316097..1316618 (+) 522 WP_005623982.1 shikimate kinase AroK -
  NYR86_RS05820 (NYR86_05820) aroB 1316635..1317723 (+) 1089 WP_279622988.1 3-dehydroquinate synthase -
  NYR86_RS05825 (NYR86_05825) - 1317727..1318563 (+) 837 WP_039197452.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NYR86_RS05830 (NYR86_05830) - 1318633..1319991 (+) 1359 WP_278225881.1 sodium-dependent transporter -
  NYR86_RS05835 (NYR86_05835) pgi 1320119..1321759 (-) 1641 WP_279622989.1 glucose-6-phosphate isomerase -
  NYR86_RS05840 (NYR86_05840) - 1321798..1322049 (-) 252 WP_039197458.1 accessory factor UbiK family protein -
  NYR86_RS05865 (NYR86_05865) aroE 1322768..1323592 (-) 825 WP_279622990.1 shikimate dehydrogenase -
  NYR86_RS05870 (NYR86_05870) - 1323598..1324152 (-) 555 WP_275217136.1 Sua5/YciO/YrdC/YwlC family protein -
  NYR86_RS05875 (NYR86_05875) - 1324145..1324678 (-) 534 WP_039197464.1 type I DNA topoisomerase -
  NYR86_RS05880 (NYR86_05880) yqfB 1324678..1324983 (-) 306 WP_279622991.1 N(4)-acetylcytidine aminohydrolase -
  NYR86_RS05885 (NYR86_05885) recX 1324976..1325431 (-) 456 WP_237610172.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47708.84 Da        Isoelectric Point: 9.4293

>NTDB_id=725751 NYR86_RS05785 WP_279622985.1 1309683..1310981(-) (comE) [Actinobacillus equuli subsp. equuli strain 2499]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKDEKAATPIAINPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=725751 NYR86_RS05785 WP_279622985.1 1309683..1310981(-) (comE) [Actinobacillus equuli subsp. equuli strain 2499]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCAACAGCGGAAGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACACAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTATAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGGAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCCGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAATGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTAGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAATGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(256-415)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.427

96.528

0.641
  comE Haemophilus influenzae 86-028NP

54.415

96.991

0.528
  comE Haemophilus influenzae Rd KW20

55.583

93.287

0.519
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.614

96.065

0.419
  pilQ Vibrio cholerae strain A1552

43.614

96.065

0.419
  pilQ Vibrio campbellii strain DS40M4

41.469

97.685

0.405