Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR66_RS05780 Genome accession   NZ_CP103829
Coordinates   1260936..1262234 (-) Length   432 a.a.
NCBI ID   WP_279455589.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 2863     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1255936..1267234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR66_RS05750 (NYR66_05755) suhB 1256109..1256921 (-) 813 WP_279438014.1 inositol-1-monophosphatase -
  NYR66_RS05755 (NYR66_05760) - 1257088..1258185 (+) 1098 WP_279455586.1 TrmH family RNA methyltransferase -
  NYR66_RS05760 (NYR66_05765) - 1258247..1258872 (-) 626 Protein_1117 LysE family transporter -
  NYR66_RS05765 (NYR66_05770) - 1258881..1259354 (-) 474 WP_279455587.1 phosphatidylglycerophosphatase A -
  NYR66_RS05770 (NYR66_05775) thiL 1259367..1260332 (-) 966 WP_279452962.1 thiamine-phosphate kinase -
  NYR66_RS05775 (NYR66_05780) nusB 1260397..1260810 (-) 414 WP_278225876.1 transcription antitermination factor NusB -
  NYR66_RS05780 (NYR66_05785) comE 1260936..1262234 (-) 1299 WP_279455589.1 type IV pilus secretin PilQ Machinery gene
  NYR66_RS05785 (NYR66_05790) - 1262246..1262650 (-) 405 WP_279438018.1 hypothetical protein -
  NYR66_RS05790 (NYR66_05795) - 1262643..1263170 (-) 528 WP_279438019.1 chromosome segregation protein -
  NYR66_RS05795 (NYR66_05800) - 1263173..1263694 (-) 522 WP_275217127.1 hypothetical protein -
  NYR66_RS05800 (NYR66_05805) - 1263676..1264362 (-) 687 WP_275217128.1 pilus assembly protein PilM -
  NYR66_RS05805 (NYR66_05810) - 1264501..1267086 (+) 2586 WP_279455590.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47770.91 Da        Isoelectric Point: 9.4018

>NTDB_id=725724 NYR66_RS05780 WP_279455589.1 1260936..1262234(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 2863]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIEYLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=725724 NYR66_RS05780 WP_279455589.1 1260936..1262234(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 2863]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAAGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACATAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATATTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCA
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAACTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCCGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAATGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAATGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.187

96.528

0.639
  comE Haemophilus influenzae 86-028NP

54.177

96.991

0.525
  comE Haemophilus influenzae Rd KW20

55.335

93.287

0.516
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.237

95.833

0.414
  pilQ Vibrio cholerae strain A1552

43.237

95.833

0.414
  pilQ Vibrio campbellii strain DS40M4

41.469

97.685

0.405