Detailed information NTG0725724

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: NYR66_RS05780
• Genome accession: NZ_CP103829
• Coordinates: 1260936..1262234 (-)
• Length: 432 a.a.
• NCBI ID: WP_279455589.1
• Uniprot ID: -
• Organism: Actinobacillus equuli subsp. haemolyticus strain 2863
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1258881..1276694	1260936..1262234	within	0

Gene organization within MGE regions

Location: 1258881..1276694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYR66_RS05765 (NYR66_05770)	-	1258881..1259354 (-)	474	WP_279455587.1	phosphatidylglycerophosphatase A	-
NYR66_RS05770 (NYR66_05775)	thiL	1259367..1260332 (-)	966	WP_279452962.1	thiamine-phosphate kinase	-
NYR66_RS05775 (NYR66_05780)	nusB	1260397..1260810 (-)	414	WP_278225876.1	transcription antitermination factor NusB	-
NYR66_RS05780 (NYR66_05785)	comE	1260936..1262234 (-)	1299	WP_279455589.1	type IV pilus secretin PilQ	Machinery gene
NYR66_RS05785 (NYR66_05790)	-	1262246..1262650 (-)	405	WP_279438018.1	hypothetical protein	-
NYR66_RS05790 (NYR66_05795)	-	1262643..1263170 (-)	528	WP_279438019.1	chromosome segregation protein	-
NYR66_RS05795 (NYR66_05800)	-	1263173..1263694 (-)	522	WP_275217127.1	hypothetical protein	-
NYR66_RS05800 (NYR66_05805)	-	1263676..1264362 (-)	687	WP_275217128.1	pilus assembly protein PilM	-
NYR66_RS05805 (NYR66_05810)	-	1264501..1267086 (+)	2586	WP_279455590.1	penicillin-binding protein 1A	-
NYR66_RS05810 (NYR66_05815)	aroK	1267350..1267871 (+)	522	WP_005623982.1	shikimate kinase AroK	-
NYR66_RS05815 (NYR66_05820)	aroB	1267888..1268976 (+)	1089	WP_039197450.1	3-dehydroquinate synthase	-
NYR66_RS05820 (NYR66_05825)	-	1268980..1269816 (+)	837	WP_039197452.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
NYR66_RS05825 (NYR66_05830)	-	1269886..1271244 (+)	1359	WP_278225881.1	sodium-dependent transporter	-
NYR66_RS05830 (NYR66_05835)	pgi	1271372..1273012 (-)	1641	WP_279455591.1	glucose-6-phosphate isomerase	-
NYR66_RS05835 (NYR66_05840)	-	1273051..1273302 (-)	252	WP_039197458.1	accessory factor UbiK family protein	-
NYR66_RS05860 (NYR66_05865)	aroE	1274019..1274843 (-)	825	WP_275217135.1	shikimate dehydrogenase	-
NYR66_RS05865 (NYR66_05870)	-	1274849..1275403 (-)	555	WP_279442864.1	Sua5/YciO/YrdC/YwlC family protein	-
NYR66_RS05870 (NYR66_05875)	-	1275396..1275929 (-)	534	WP_279442865.1	type I DNA topoisomerase	-
NYR66_RS05875 (NYR66_05880)	yqfB	1275929..1276234 (-)	306	WP_275217138.1	N(4)-acetylcytidine aminohydrolase	-
NYR66_RS05880 (NYR66_05885)	recX	1276227..1276682 (-)	456	WP_237610172.1	recombination regulator RecX	-

Sequence

Protein

• Length: 432 a.a.
• Molecular weight: 47770.91 Da
• Isoelectric Point: 9.4018

>NTDB_id=725724 NYR66_RS05780 WP_279455589.1 1260936..1262234(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 2863]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIEYLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide

• Length: 1299 bp

>NTDB_id=725724 NYR66_RS05780 WP_279455589.1 1260936..1262234(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 2863]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAAGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACATAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATATTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCA
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAACTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCCGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAATGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAATGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

• Bacterial type II/III secretion system short domain (Secretin_N) (112-177)

• Bacterial type II and III secretion system protein (Secretin) (256-415)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Glaesserella parasuis strain SC1401	66.187	96.528	0.639
comE	Haemophilus influenzae 86-028NP	54.177	96.991	0.525
comE	Haemophilus influenzae Rd KW20	55.335	93.287	0.516
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	43.237	95.833	0.414
pilQ	Vibrio cholerae strain A1552	43.237	95.833	0.414
pilQ	Vibrio campbellii strain DS40M4	41.469	97.685	0.405