Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR81_RS02065 Genome accession   NZ_CP103826
Coordinates   444233..445528 (+) Length   431 a.a.
NCBI ID   WP_279479564.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3113     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 429935..444222 444233..445528 flank 11


Gene organization within MGE regions


Location: 429935..445528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR81_RS01960 (NYR81_01960) yqfB 429947..430252 (+) 306 WP_275217138.1 N(4)-acetylcytidine aminohydrolase -
  NYR81_RS01965 (NYR81_01965) - 430252..430785 (+) 534 WP_279439262.1 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
  NYR81_RS01970 (NYR81_01970) - 430778..431332 (+) 555 WP_279441494.1 Sua5/YciO/YrdC/YwlC family protein -
  NYR81_RS01975 (NYR81_01975) aroE 431339..432163 (+) 825 WP_279441493.1 shikimate dehydrogenase -
  NYR81_RS02010 (NYR81_02010) - 433163..433414 (+) 252 WP_039197458.1 accessory factor UbiK family protein -
  NYR81_RS02015 (NYR81_02015) pgi 433453..435093 (+) 1641 WP_279479545.1 glucose-6-phosphate isomerase -
  NYR81_RS02020 (NYR81_02020) - 435221..436579 (-) 1359 WP_279441491.1 sodium-dependent transporter -
  NYR81_RS02025 (NYR81_02025) - 436649..437487 (-) 839 Protein_382 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NYR81_RS02030 (NYR81_02030) aroB 437491..438580 (-) 1090 Protein_383 3-dehydroquinate synthase -
  NYR81_RS02035 (NYR81_02035) aroK 438597..439118 (-) 522 WP_279479547.1 shikimate kinase AroK -
  NYR81_RS02040 (NYR81_02040) - 439382..441967 (-) 2586 WP_279479550.1 penicillin-binding protein 1A -
  NYR81_RS02045 (NYR81_02045) - 442106..442792 (+) 687 WP_279436623.1 pilus assembly protein PilM -
  NYR81_RS02050 (NYR81_02050) - 442774..443295 (+) 522 WP_279436621.1 hypothetical protein -
  NYR81_RS02055 (NYR81_02055) - 443298..443825 (+) 528 WP_279436620.1 chromosome segregation protein -
  NYR81_RS02060 (NYR81_02060) - 443818..444222 (+) 405 WP_279441488.1 hypothetical protein -
  NYR81_RS02065 (NYR81_02065) comE 444233..445528 (+) 1296 WP_279479564.1 type IV pilus secretin PilQ Machinery gene

Sequence

Protein


Download         Length: 431 a.a.        Molecular weight: 47715.68 Da        Isoelectric Point: 8.3533

>NTDB_id=725644 NYR81_RS02065 WP_279479564.1 444233..445528(+) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3113]
MKKAILLLFFIFSSVTVYAFSISLKNAPTAEILSYLAEEHGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTTAY
ENQIYFIGHKKDEKAATIGVNSELLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPESM
KNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRSGMFSPTNGHHKVVGSLEANGLPNTNHLNVNFPVNNAASIALQ
VAKINGRVLDLELTALEQENDVEIIADPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDNQ
ILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRIS
KRELVIFVTPYIVKSEKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1296 bp        

>NTDB_id=725644 NYR81_RS02065 WP_279479564.1 444233..445528(+) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3113]
ATGAAGAAAGCGATTTTGTTACTCTTTTTTATATTTTCATCCGTTACTGTGTATGCATTTTCTATCTCGTTAAAAAATGC
ACCGACCGCAGAAATTCTGAGTTATTTAGCGGAAGAGCACGGAAAAAATATTGTGCTAAGCGACAATATTGAAACGAATA
CCACATTAAGAATTGAAAATAGTGATTTTGATAGTGTTTTAAAAAGTATCACTCGAGCGAATAAATTGACGACAGCATAT
GAGAACCAAATCTATTTTATTGGCCATAAAAAAGATGAAAAGGCTGCTACTATAGGCGTAAATTCTGAGTTGTTAAAGCC
CAAGCTCATTACTAAAACCATCAAATTAGATTATGCCAAAGCGGCGGAAGTGATTGAATCTTTAACCAAAGGCAGCGGAA
ACTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCGATG
AAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAATAAG
TAGTGAAAATTTGCAAGAGCTTGGGGTACGCTCGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGTCGGTTCGC
TTGAAGCAAATGGACTACCGAATACTAACCATTTAAACGTAAATTTTCCGGTAAATAATGCTGCATCTATTGCGCTACAA
GTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGCCGA
CCCTCGTTTACTGACAACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACCGTA
AAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCACATATTTCAAATGATAATCAA
ATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGATAA
ACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCGCAA
AAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATCAGT
AAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGAAAAACAGCAAATTTCCTCGCATTCTTCACAGAA
ATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(111-176)

Bacterial type II and III secretion system protein (Secretin)

(255-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.187

96.752

0.64
  comE Haemophilus influenzae Rd KW20

55.5

92.807

0.515
  comE Haemophilus influenzae 86-028NP

55.112

93.039

0.513
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.754

96.056

0.411
  pilQ Vibrio cholerae strain A1552

42.754

96.056

0.411
  pilQ Vibrio campbellii strain DS40M4

41.469

97.912

0.406