Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR77_RS02045 Genome accession   NZ_CP103822
Coordinates   450504..451802 (-) Length   432 a.a.
NCBI ID   WP_279441053.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3617     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445504..456802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR77_RS02015 (NYR77_02010) suhB 445677..446489 (-) 813 WP_279436613.1 inositol-1-monophosphatase -
  NYR77_RS02020 (NYR77_02015) - 446656..447753 (+) 1098 WP_279441051.1 TrmH family RNA methyltransferase -
  NYR77_RS02025 (NYR77_02020) - 447815..448440 (-) 626 Protein_386 LysE family transporter -
  NYR77_RS02030 (NYR77_02025) - 448449..448922 (-) 474 WP_279436616.1 phosphatidylglycerophosphatase A -
  NYR77_RS02035 (NYR77_02030) thiL 448935..449900 (-) 966 WP_279441052.1 thiamine-phosphate kinase -
  NYR77_RS02040 (NYR77_02035) nusB 449965..450378 (-) 414 WP_278225876.1 transcription antitermination factor NusB -
  NYR77_RS02045 (NYR77_02040) comE 450504..451802 (-) 1299 WP_279441053.1 type IV pilus secretin PilQ Machinery gene
  NYR77_RS02050 (NYR77_02045) - 451814..452218 (-) 405 WP_279441054.1 hypothetical protein -
  NYR77_RS02055 (NYR77_02050) - 452211..452738 (-) 528 WP_279441055.1 chromosome segregation protein -
  NYR77_RS02060 (NYR77_02055) - 452741..453262 (-) 522 WP_279441056.1 hypothetical protein -
  NYR77_RS02065 (NYR77_02060) - 453244..453930 (-) 687 WP_279441057.1 pilus assembly protein PilM -
  NYR77_RS02070 (NYR77_02065) - 454069..456654 (+) 2586 WP_279441058.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47662.76 Da        Isoelectric Point: 9.4293

>NTDB_id=725566 NYR77_RS02045 WP_279441053.1 450504..451802(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3617]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRVPILGSIPVLKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=725566 NYR77_RS02045 WP_279441053.1 450504..451802(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3617]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAGGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACACAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCTGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATTGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAACGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTTGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTCCCAATTTTAGGTTCAATTCCGGTACTTAAAAAATTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(256-415)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.427

96.528

0.641
  comE Haemophilus influenzae 86-028NP

54.415

96.991

0.528
  comE Haemophilus influenzae Rd KW20

55.583

93.287

0.519
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.096

96.065

0.424
  pilQ Vibrio cholerae strain A1552

44.096

96.065

0.424
  pilQ Vibrio campbellii strain DS40M4

41.943

97.685

0.41