Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR75_RS05785 Genome accession   NZ_CP103820
Coordinates   1259990..1261288 (-) Length   432 a.a.
NCBI ID   WP_279471414.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 3820     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1262227..1275747 1259990..1261288 flank 939


Gene organization within MGE regions


Location: 1259990..1275747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR75_RS05785 (NYR75_05790) comE 1259990..1261288 (-) 1299 WP_279471414.1 type IV pilus secretin PilQ Machinery gene
  NYR75_RS05790 (NYR75_05795) - 1261300..1261704 (-) 405 WP_279471415.1 hypothetical protein -
  NYR75_RS05795 (NYR75_05800) - 1261697..1262224 (-) 528 WP_279438019.1 chromosome segregation protein -
  NYR75_RS05800 (NYR75_05805) - 1262227..1262748 (-) 522 WP_279442859.1 hypothetical protein -
  NYR75_RS05805 (NYR75_05810) - 1262730..1263416 (-) 687 WP_039197447.1 hypothetical protein -
  NYR75_RS05810 (NYR75_05815) - 1263555..1266140 (+) 2586 WP_279471416.1 penicillin-binding protein 1A -
  NYR75_RS05815 (NYR75_05820) aroK 1266404..1266925 (+) 522 WP_005623982.1 shikimate kinase AroK -
  NYR75_RS05820 (NYR75_05825) aroB 1266942..1268030 (+) 1089 WP_039197450.1 3-dehydroquinate synthase -
  NYR75_RS05825 (NYR75_05830) - 1268034..1268870 (+) 837 WP_039197452.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NYR75_RS05830 (NYR75_05835) - 1268940..1270298 (+) 1359 WP_039197454.1 sodium-dependent transporter -
  NYR75_RS05835 (NYR75_05840) pgi 1270426..1272066 (-) 1641 WP_279471417.1 glucose-6-phosphate isomerase -
  NYR75_RS05840 (NYR75_05845) - 1272105..1272356 (-) 252 WP_039197458.1 accessory factor UbiK family protein -
  NYR75_RS05865 (NYR75_05870) aroE 1273072..1273896 (-) 825 WP_279471418.1 shikimate dehydrogenase -
  NYR75_RS05870 (NYR75_05875) - 1273902..1274456 (-) 555 WP_279471419.1 Sua5/YciO/YrdC/YwlC family protein -
  NYR75_RS05875 (NYR75_05880) - 1274449..1274982 (-) 534 WP_039197464.1 type I DNA topoisomerase -
  NYR75_RS05880 (NYR75_05885) yqfB 1274982..1275287 (-) 306 WP_275217138.1 N(4)-acetylcytidine aminohydrolase -
  NYR75_RS05885 (NYR75_05890) recX 1275280..1275735 (-) 456 WP_237610172.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47736.85 Da        Isoelectric Point: 9.2401

>NTDB_id=725519 NYR75_RS05785 WP_279471414.1 1259990..1261288(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3820]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLDSAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKIAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=725519 NYR75_RS05785 WP_279471414.1 1259990..1261288(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3820]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAGGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTGGACAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACATAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAATTGCCGGTT
CGCTTGAAGCAAATGGATTACCGAATACTAACCATTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTAGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCACATATTTCAAATGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.427

96.528

0.641
  comE Haemophilus influenzae 86-028NP

54.177

96.991

0.525
  comE Haemophilus influenzae Rd KW20

55.335

93.287

0.516
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.614

96.065

0.419
  pilQ Vibrio cholerae strain A1552

43.614

96.065

0.419
  pilQ Vibrio campbellii strain DS40M4

41.469

97.685

0.405