Detailed information NTG0725501

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: NYR74_RS10685
• Genome accession: NZ_CP103819
• Coordinates: 2264144..2265442 (+)
• Length: 432 a.a.
• NCBI ID: WP_279452964.1
• Uniprot ID: -
• Organism: Actinobacillus equuli subsp. haemolyticus strain 3873
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2250134..2268131	2264144..2265442	within	0

Gene organization within MGE regions

Location: 2250134..2268131

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYR74_RS10590 (NYR74_10595)	yqfB	2250146..2250451 (+)	306	WP_279443862.1	N(4)-acetylcytidine aminohydrolase	-
NYR74_RS10595 (NYR74_10600)	-	2250451..2250983 (+)	533	Protein_2031	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
NYR74_RS10600 (NYR74_10605)	-	2250976..2251530 (+)	555	WP_275217136.1	Sua5/YciO/YrdC/YwlC family protein	-
NYR74_RS10605 (NYR74_10610)	aroE	2251536..2252360 (+)	825	WP_279452970.1	shikimate dehydrogenase	-
NYR74_RS10630 (NYR74_10635)	-	2253076..2253327 (+)	252	WP_039197458.1	accessory factor UbiK family protein	-
NYR74_RS10635 (NYR74_10640)	pgi	2253366..2255007 (+)	1642	Protein_2035	glucose-6-phosphate isomerase	-
NYR74_RS10640 (NYR74_10645)	-	2255135..2256493 (-)	1359	WP_279452968.1	sodium-dependent transporter	-
NYR74_RS10645 (NYR74_10650)	-	2256563..2257399 (-)	837	WP_039197452.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
NYR74_RS10650 (NYR74_10655)	aroB	2257403..2258491 (-)	1089	WP_279452967.1	3-dehydroquinate synthase	-
NYR74_RS10655 (NYR74_10660)	aroK	2258508..2259029 (-)	522	WP_279452966.1	shikimate kinase AroK	-
NYR74_RS10660 (NYR74_10665)	-	2259293..2261878 (-)	2586	WP_279436624.1	penicillin-binding protein 1A	-
NYR74_RS10665 (NYR74_10670)	-	2262017..2262703 (+)	687	WP_279436623.1	pilus assembly protein PilM	-
NYR74_RS10670 (NYR74_10675)	-	2262685..2263206 (+)	522	WP_279436621.1	hypothetical protein	-
NYR74_RS10675 (NYR74_10680)	-	2263209..2263736 (+)	528	WP_279436620.1	chromosome segregation protein	-
NYR74_RS10680 (NYR74_10685)	-	2263729..2264133 (+)	405	WP_279436619.1	hypothetical protein	-
NYR74_RS10685 (NYR74_10690)	comE	2264144..2265442 (+)	1299	WP_279452964.1	type IV pilus secretin PilQ	Machinery gene
NYR74_RS10690 (NYR74_10695)	nusB	2265568..2265981 (+)	414	WP_279452963.1	transcription antitermination factor NusB	-
NYR74_RS10695 (NYR74_10700)	thiL	2266046..2267011 (+)	966	WP_279452962.1	thiamine-phosphate kinase	-
NYR74_RS10700 (NYR74_10705)	-	2267024..2267497 (+)	474	WP_279452961.1	phosphatidylglycerophosphatase A	-
NYR74_RS10705 (NYR74_10710)	-	2267506..2268131 (+)	626	Protein_2049	LysE family transporter	-

Sequence

Protein

• Length: 432 a.a.
• Molecular weight: 47690.77 Da
• Isoelectric Point: 9.5571

>NTDB_id=725501 NYR74_RS10685 WP_279452964.1 2264144..2265442(+) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3873]
MKKAILLLFFIFSSVTVYAFSISLKNAPTAEILSYLAEEHGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTTAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIGSLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPANGHHKIAGSLEANGLPNTNHLNVNFPVNNAASLAL
QVAKINGRVLDLELTALEQENNVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide

• Length: 1299 bp

>NTDB_id=725501 NYR74_RS10685 WP_279452964.1 2264144..2265442(+) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3873]
ATGAAGAAAGCGATTTTGTTACTCTTTTTTATATTTTCATCCGTTACTGTGTATGCATTTTCTATCTCGTTAAAAAATGC
ACCGACCGCAGAAATTCTGAGTTATTTAGCGGAAGAGCACGGAAAAAATATTGTGCTAAGCGACAATATTGAAACGAATA
CCACATTAAGAATTGAAAATAGTGATTTTGATAGTGTTTTAAAAAGTATCACTCGAGCGAATAAATTGACGACAGCATAT
GAGAACCAAATCTATTTTATTGGACACAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGGATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGGCAAACGGACATCATAAAATTGCCGGTT
CGCTTGAAGCAAATGGGCTACCGAATACTAATCATTTAAACGTAAATTTTCCGGTAAATAATGCTGCTTCACTTGCGCTA
CAAGTGGCAAAAATTAATGGGCGAGTGCTTGATTTGGAACTCACCGCTTTGGAACAAGAAAATAATGTAGAAATTATTGC
CAGCCCCCGTTTATTGACTACCAATAAGAAGCCGGCGAGTATTAAGCAAGGGACAGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCACATATTTCAAATGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATAGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (256-415)

• Bacterial type II/III secretion system short domain (Secretin_N) (112-177)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Glaesserella parasuis strain SC1401	66.268	96.759	0.641
comE	Haemophilus influenzae Rd KW20	55.583	93.287	0.519
comE	Haemophilus influenzae 86-028NP	55.198	93.519	0.516
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	42.892	96.065	0.412
pilQ	Vibrio cholerae strain A1552	42.892	96.065	0.412
pilQ	Vibrio campbellii strain DS40M4	40.995	97.685	0.4