Detailed information NTG0725470

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: NYR73_RS06020
• Genome accession: NZ_CP103818
• Coordinates: 1326731..1328029 (-)
• Length: 432 a.a.
• NCBI ID: WP_279452964.1
• Uniprot ID: -
• Organism: Actinobacillus equuli subsp. haemolyticus strain 3874
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1324676..1342487	1326731..1328029	within	0

Gene organization within MGE regions

Location: 1324676..1342487

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYR73_RS06005 (NYR73_06010)	-	1324676..1325149 (-)	474	WP_279452961.1	phosphatidylglycerophosphatase A	-
NYR73_RS06010 (NYR73_06015)	thiL	1325162..1326127 (-)	966	WP_279452962.1	thiamine-phosphate kinase	-
NYR73_RS06015 (NYR73_06020)	nusB	1326192..1326605 (-)	414	WP_279452963.1	transcription antitermination factor NusB	-
NYR73_RS06020 (NYR73_06025)	comE	1326731..1328029 (-)	1299	WP_279452964.1	type IV pilus secretin PilQ	Machinery gene
NYR73_RS06025 (NYR73_06030)	-	1328040..1328444 (-)	405	WP_279436619.1	hypothetical protein	-
NYR73_RS06030 (NYR73_06035)	-	1328437..1328796 (-)	360	WP_279452965.1	chromosome segregation protein	-
NYR73_RS06035 (NYR73_06040)	-	1328967..1329488 (-)	522	WP_279436621.1	hypothetical protein	-
NYR73_RS06040 (NYR73_06045)	-	1329470..1330156 (-)	687	WP_279436623.1	pilus assembly protein PilM	-
NYR73_RS06045 (NYR73_06050)	-	1330295..1332880 (+)	2586	WP_279436624.1	penicillin-binding protein 1A	-
NYR73_RS06050 (NYR73_06055)	aroK	1333144..1333665 (+)	522	WP_279452966.1	shikimate kinase AroK	-
NYR73_RS06055 (NYR73_06060)	aroB	1333682..1334770 (+)	1089	WP_279452967.1	3-dehydroquinate synthase	-
NYR73_RS06060 (NYR73_06065)	-	1334774..1335610 (+)	837	WP_039197452.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
NYR73_RS06065 (NYR73_06070)	-	1335680..1337038 (+)	1359	WP_279452968.1	sodium-dependent transporter	-
NYR73_RS06070 (NYR73_06075)	pgi	1337166..1338806 (-)	1641	WP_279452969.1	glucose-6-phosphate isomerase	-
NYR73_RS06075 (NYR73_06080)	-	1338845..1339096 (-)	252	WP_039197458.1	accessory factor UbiK family protein	-
NYR73_RS06100 (NYR73_06105)	aroE	1339812..1340636 (-)	825	WP_279452970.1	shikimate dehydrogenase	-
NYR73_RS06105 (NYR73_06110)	-	1340642..1341196 (-)	555	WP_275217136.1	Sua5/YciO/YrdC/YwlC family protein	-
NYR73_RS06110 (NYR73_06115)	-	1341189..1341722 (-)	534	WP_279452971.1	type I DNA topoisomerase	-
NYR73_RS06115 (NYR73_06120)	yqfB	1341722..1342027 (-)	306	WP_279443862.1	N(4)-acetylcytidine aminohydrolase	-
NYR73_RS06120 (NYR73_06125)	recX	1342020..1342475 (-)	456	WP_279452972.1	recombination regulator RecX	-

Sequence

Protein

• Length: 432 a.a.
• Molecular weight: 47690.77 Da
• Isoelectric Point: 9.5571

>NTDB_id=725470 NYR73_RS06020 WP_279452964.1 1326731..1328029(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3874]
MKKAILLLFFIFSSVTVYAFSISLKNAPTAEILSYLAEEHGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTTAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIGSLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPANGHHKIAGSLEANGLPNTNHLNVNFPVNNAASLAL
QVAKINGRVLDLELTALEQENNVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide

• Length: 1299 bp

>NTDB_id=725470 NYR73_RS06020 WP_279452964.1 1326731..1328029(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 3874]
ATGAAGAAAGCGATTTTGTTACTCTTTTTTATATTTTCATCCGTTACTGTGTATGCATTTTCTATCTCGTTAAAAAATGC
ACCGACCGCAGAAATTCTGAGTTATTTAGCGGAAGAGCACGGAAAAAATATTGTGCTAAGCGACAATATTGAAACGAATA
CCACATTAAGAATTGAAAATAGTGATTTTGATAGTGTTTTAAAAAGTATCACTCGAGCGAATAAATTGACGACAGCATAT
GAGAACCAAATCTATTTTATTGGACACAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGGATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCGGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGGCAAACGGACATCATAAAATTGCCGGTT
CGCTTGAAGCAAATGGGCTACCGAATACTAATCATTTAAACGTAAATTTTCCGGTAAATAATGCTGCTTCACTTGCGCTA
CAAGTGGCAAAAATTAATGGGCGAGTGCTTGATTTGGAACTCACCGCTTTGGAACAAGAAAATAATGTAGAAATTATTGC
CAGCCCCCGTTTATTGACTACCAATAAGAAGCCGGCGAGTATTAAGCAAGGGACAGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCACATATTTCAAATGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATAGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (256-415)

• Bacterial type II/III secretion system short domain (Secretin_N) (112-177)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Glaesserella parasuis strain SC1401	66.268	96.759	0.641
comE	Haemophilus influenzae Rd KW20	55.583	93.287	0.519
comE	Haemophilus influenzae 86-028NP	55.198	93.519	0.516
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	42.892	96.065	0.412
pilQ	Vibrio cholerae strain A1552	42.892	96.065	0.412
pilQ	Vibrio campbellii strain DS40M4	40.995	97.685	0.4