Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR70_RS05715 Genome accession   NZ_CP103816
Coordinates   1286304..1287602 (-) Length   432 a.a.
NCBI ID   WP_279476772.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. equuli strain 4026     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1288541..1302052 1286304..1287602 flank 939


Gene organization within MGE regions


Location: 1286304..1302052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR70_RS05715 (NYR70_05715) comE 1286304..1287602 (-) 1299 WP_279476772.1 type IV pilus secretin PilQ Machinery gene
  NYR70_RS05720 (NYR70_05720) - 1287614..1288018 (-) 405 WP_278225877.1 hypothetical protein -
  NYR70_RS05725 (NYR70_05725) - 1288011..1288538 (-) 528 WP_279438019.1 chromosome segregation protein -
  NYR70_RS05730 (NYR70_05730) - 1288541..1289062 (-) 522 WP_279442859.1 hypothetical protein -
  NYR70_RS05735 (NYR70_05735) - 1289044..1289730 (-) 687 WP_039197447.1 hypothetical protein -
  NYR70_RS05740 (NYR70_05740) - 1289869..1292454 (+) 2586 WP_279438021.1 penicillin-binding protein 1A -
  NYR70_RS05745 (NYR70_05745) aroK 1292718..1293239 (+) 522 WP_005623982.1 shikimate kinase AroK -
  NYR70_RS05750 (NYR70_05750) aroB 1293256..1294344 (+) 1089 WP_039197450.1 3-dehydroquinate synthase -
  NYR70_RS05755 (NYR70_05755) - 1294348..1295184 (+) 837 WP_039197452.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NYR70_RS05760 (NYR70_05760) - 1295254..1296612 (+) 1359 WP_278225881.1 sodium-dependent transporter -
  NYR70_RS05765 (NYR70_05765) pgi 1296741..1298381 (-) 1641 WP_279476773.1 glucose-6-phosphate isomerase -
  NYR70_RS05770 (NYR70_05770) - 1298420..1298671 (-) 252 WP_039197458.1 accessory factor UbiK family protein -
  NYR70_RS05795 (NYR70_05795) aroE 1299389..1300213 (-) 825 WP_279441047.1 shikimate dehydrogenase -
  NYR70_RS05800 (NYR70_05800) - 1300219..1300773 (-) 555 WP_279439261.1 Sua5/YciO/YrdC/YwlC family protein -
  NYR70_RS05805 (NYR70_05805) - 1300766..1301299 (-) 534 WP_279476774.1 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
  NYR70_RS05810 (NYR70_05810) yqfB 1301299..1301604 (-) 306 WP_275217138.1 N(4)-acetylcytidine aminohydrolase -
  NYR70_RS05815 (NYR70_05815) recX 1301597..1302052 (-) 456 WP_237610172.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47675.80 Da        Isoelectric Point: 9.5705

>NTDB_id=725418 NYR70_RS05715 WP_279476772.1 1286304..1287602(-) (comE) [Actinobacillus equuli subsp. equuli strain 4026]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKNEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRIPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=725418 NYR70_RS05715 WP_279476772.1 1286304..1287602(-) (comE) [Actinobacillus equuli subsp. equuli strain 4026]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAAGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACACAAAAAAAATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCTGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAACGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATACCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.906

96.528

0.646
  comE Haemophilus influenzae 86-028NP

54.415

96.991

0.528
  comE Haemophilus influenzae Rd KW20

55.583

93.287

0.519
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.614

96.065

0.419
  pilQ Vibrio cholerae strain A1552

43.614

96.065

0.419
  pilQ Vibrio campbellii strain DS40M4

41.469

97.685

0.405