Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NYR69_RS05770 Genome accession   NZ_CP103815
Coordinates   1281034..1282332 (-) Length   432 a.a.
NCBI ID   WP_279438017.1    Uniprot ID   -
Organism   Actinobacillus equuli subsp. haemolyticus strain 4038     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1283271..1296782 1281034..1282332 flank 939


Gene organization within MGE regions


Location: 1281034..1296782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR69_RS05770 (NYR69_05775) comE 1281034..1282332 (-) 1299 WP_279438017.1 type IV pilus secretin PilQ Machinery gene
  NYR69_RS05775 (NYR69_05780) - 1282344..1282748 (-) 405 WP_279438018.1 hypothetical protein -
  NYR69_RS05780 (NYR69_05785) - 1282741..1283268 (-) 528 WP_279438019.1 chromosome segregation protein -
  NYR69_RS05785 (NYR69_05790) - 1283271..1283792 (-) 522 WP_275217127.1 hypothetical protein -
  NYR69_RS05790 (NYR69_05795) - 1283774..1284460 (-) 687 WP_275217128.1 pilus assembly protein PilM -
  NYR69_RS05795 (NYR69_05800) - 1284599..1287184 (+) 2586 WP_279438021.1 penicillin-binding protein 1A -
  NYR69_RS05800 (NYR69_05805) aroK 1287448..1287969 (+) 522 WP_005623982.1 shikimate kinase AroK -
  NYR69_RS05805 (NYR69_05810) aroB 1287986..1289074 (+) 1089 WP_039197450.1 3-dehydroquinate synthase -
  NYR69_RS05810 (NYR69_05815) - 1289078..1289914 (+) 837 WP_279438022.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NYR69_RS05815 (NYR69_05820) - 1289984..1291342 (+) 1359 WP_279438023.1 sodium-dependent transporter -
  NYR69_RS05820 (NYR69_05825) pgi 1291471..1293111 (-) 1641 WP_279438024.1 glucose-6-phosphate isomerase -
  NYR69_RS05825 (NYR69_05830) - 1293150..1293401 (-) 252 WP_039197458.1 accessory factor UbiK family protein -
  NYR69_RS05850 (NYR69_05855) aroE 1294119..1294943 (-) 825 WP_279438025.1 shikimate dehydrogenase -
  NYR69_RS05855 (NYR69_05860) - 1294949..1295503 (-) 555 WP_279438026.1 Sua5/YciO/YrdC/YwlC family protein -
  NYR69_RS05860 (NYR69_05865) - 1295496..1296029 (-) 534 WP_279438027.1 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
  NYR69_RS05865 (NYR69_05870) yqfB 1296029..1296334 (-) 306 WP_279438028.1 N(4)-acetylcytidine aminohydrolase -
  NYR69_RS05870 (NYR69_05875) recX 1296327..1296782 (-) 456 WP_279438030.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47694.82 Da        Isoelectric Point: 9.4293

>NTDB_id=725391 NYR69_RS05770 WP_279438017.1 1281034..1282332(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 4038]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLSSAY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRMPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=725391 NYR69_RS05770 WP_279438017.1 1281034..1282332(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 4038]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAAGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTCAGCAGTGCGTAT
GAAAACCAGATCTATTTTATTGGACATAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCA
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAACTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCCGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAATGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTGGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAATGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAATGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(256-415)

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.427

96.528

0.641
  comE Haemophilus influenzae 86-028NP

54.415

96.991

0.528
  comE Haemophilus influenzae Rd KW20

55.583

93.287

0.519
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.614

96.065

0.419
  pilQ Vibrio cholerae strain A1552

43.614

96.065

0.419
  pilQ Vibrio campbellii strain DS40M4

41.469

97.685

0.405