Detailed information NTG0725364

Bioinformatically predicted

Overview

• Name: comE
• Type: Machinery gene
• Locus tag: NYR68_RS06140
• Genome accession: NZ_CP103814
• Coordinates: 1335856..1337154 (-)
• Length: 432 a.a.
• NCBI ID: WP_279621941.1
• Uniprot ID: -
• Organism: Actinobacillus equuli subsp. haemolyticus strain 4091
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1333801..1351614	1335856..1337154	within	0

Gene organization within MGE regions

Location: 1333801..1351614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYR68_RS06125 (NYR68_06130)	-	1333801..1334274 (-)	474	WP_279621939.1	phosphatidylglycerophosphatase A	-
NYR68_RS06130 (NYR68_06135)	thiL	1334287..1335252 (-)	966	WP_279621940.1	thiamine-phosphate kinase	-
NYR68_RS06135 (NYR68_06140)	nusB	1335317..1335730 (-)	414	WP_275217123.1	transcription antitermination factor NusB	-
NYR68_RS06140 (NYR68_06145)	comE	1335856..1337154 (-)	1299	WP_279621941.1	type IV pilus secretin PilQ	Machinery gene
NYR68_RS06145 (NYR68_06150)	-	1337166..1337570 (-)	405	WP_278225877.1	hypothetical protein	-
NYR68_RS06150 (NYR68_06155)	-	1337563..1338090 (-)	528	WP_279621942.1	chromosome segregation protein	-
NYR68_RS06155 (NYR68_06160)	-	1338093..1338614 (-)	522	WP_279441056.1	hypothetical protein	-
NYR68_RS06160 (NYR68_06165)	-	1338596..1339282 (-)	687	WP_279621943.1	pilus assembly protein PilM	-
NYR68_RS06165 (NYR68_06170)	-	1339421..1342006 (+)	2586	WP_279621944.1	penicillin-binding protein 1A	-
NYR68_RS06170 (NYR68_06175)	aroK	1342270..1342791 (+)	522	WP_005623982.1	shikimate kinase AroK	-
NYR68_RS06175 (NYR68_06180)	aroB	1342808..1343896 (+)	1089	WP_039197450.1	3-dehydroquinate synthase	-
NYR68_RS06180 (NYR68_06185)	-	1343900..1344736 (+)	837	WP_039197452.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
NYR68_RS06185 (NYR68_06190)	-	1344806..1346164 (+)	1359	WP_039197454.1	sodium-dependent transporter	-
NYR68_RS06190 (NYR68_06195)	pgi	1346292..1347932 (-)	1641	WP_279621945.1	glucose-6-phosphate isomerase	-
NYR68_RS06195 (NYR68_06200)	-	1347971..1348222 (-)	252	WP_039197458.1	accessory factor UbiK family protein	-
NYR68_RS06220 (NYR68_06225)	aroE	1348939..1349763 (-)	825	WP_279621946.1	shikimate dehydrogenase	-
NYR68_RS06225 (NYR68_06230)	-	1349769..1350323 (-)	555	WP_279442864.1	Sua5/YciO/YrdC/YwlC family protein	-
NYR68_RS06230 (NYR68_06235)	-	1350316..1350849 (-)	534	WP_279442865.1	type I DNA topoisomerase	-
NYR68_RS06235 (NYR68_06240)	yqfB	1350849..1351154 (-)	306	WP_275217138.1	N(4)-acetylcytidine aminohydrolase	-
NYR68_RS06240 (NYR68_06245)	recX	1351147..1351602 (-)	456	WP_237610172.1	recombination regulator RecX	-

Sequence

Protein

• Length: 432 a.a.
• Molecular weight: 47718.82 Da
• Isoelectric Point: 9.2401

>NTDB_id=725364 NYR68_RS06140 WP_279621941.1 1335856..1337154(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 4091]
MRRVILLLLLILTPVMANTFSISLKNAPTAEVLSYLAEEYGKNIVLSGNIETNTTLRIENSDFDSVLKSITRANKLDSVY
ENQIYFIGHKKDEKAATPIAVNPDVLKPKLITKTIKLDYAKAAEVIESLTKGSGNFLSENGYLHFDDRSNSLIIKDSPES
MKNIVKLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGMFSPTNGHHKVAGSLEANGLPNTNHLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENDVEIIASPRLLTTNKKPASIKQGTEIPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDN
QILLDLVVTQNSPNSTSSTVHGLVTIDKQELNTQVFAKHGETIVLGGIFQHLTAKGEDRVPILGSIPVIKKLFSHSSDRI
SKRELVIFVTPYIVKSGKQQISSHSSQKLPPK

Nucleotide

• Length: 1299 bp

>NTDB_id=725364 NYR68_RS06140 WP_279621941.1 1335856..1337154(-) (comE) [Actinobacillus equuli subsp. haemolyticus strain 4091]
ATGCGAAGAGTGATTTTGTTGCTCCTTTTGATATTAACGCCGGTTATGGCAAATACTTTTTCTATCTCGCTGAAAAATGC
GCCGACAGCGGAGGTTTTAAGTTATTTAGCCGAAGAATACGGCAAAAATATTGTGTTAAGCGGCAATATTGAAACAAATA
CTACATTAAGAATTGAAAATAGCGATTTTGATAGCGTGTTAAAAAGTATTACTCGAGCAAATAAGCTGGACAGTGTGTAT
GAAAACCAGATCTATTTTATTGGACATAAAAAAGATGAGAAAGCGGCTACGCCAATAGCTGTAAATCCGGATGTATTGAA
ACCTAAGTTGATTACTAAGACAATAAAATTAGATTATGCGAAGGCAGCGGAAGTGATTGAATCTTTAACCAAAGGTAGTG
GTAATTTTTTATCGGAAAACGGCTATCTACATTTTGATGATCGTAGTAATAGTTTGATAATTAAAGATAGCCCAGAATCG
ATGAAAAATATCGTGAAATTAATTAGAAATCTGGATAAACCGACTGAGCAGATTGCGATTGAAGCAAGGATAGTCACAAT
AAGTAGTGAAAATTTGCAAGAGCTTGGGGTACGCTGGGGAATGTTTTCTCCGACAAACGGACATCATAAAGTCGCTGGTT
CGCTTGAAGCGAATGGACTACCGAATACTAACCATTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCGCTA
CAAGTAGCAAAAATTAATGGACGAGTGCTTGATTTGGAATTAACCGCTTTAGAGCAAGAAAATGATGTGGAAATTATTGC
CAGCCCTCGTTTACTGACCACTAATAAGAAACCGGCGAGTATTAAGCAAGGGACTGAAATTCCGTATGTGCTTTATAACC
GTAAAGATGAAGTGAAAAATATCGAATTTAAAGAAGCCGTTTTAGGGCTACAGGTCACGCCGCATATTTCAAACGATAAT
CAAATTTTGCTTGATTTGGTGGTGACACAAAATTCGCCGAATTCAACCAGTTCGACAGTTCATGGTTTAGTAACGATTGA
TAAACAGGAATTAAATACGCAAGTATTCGCTAAGCATGGTGAAACTATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTGCCGATTTTAGGTTCAATTCCGGTCATTAAAAAGTTATTTAGCCATTCCAGTGATAGGATC
AGTAAGCGCGAATTAGTTATTTTCGTTACACCTTATATTGTTAAAAGTGGAAAACAGCAAATTTCCTCGCATTCTTCACA
GAAATTACCGCCAAAATAG

Domains

Predicted by InterproScan.

• Bacterial type II/III secretion system short domain (Secretin_N) (112-177)

• Bacterial type II and III secretion system protein (Secretin) (256-415)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Glaesserella parasuis strain SC1401	66.667	96.528	0.644
comE	Haemophilus influenzae 86-028NP	54.654	96.991	0.53
comE	Haemophilus influenzae Rd KW20	55.831	93.287	0.521
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	44.096	96.065	0.424
pilQ	Vibrio cholerae strain A1552	44.096	96.065	0.424
pilQ	Vibrio campbellii strain DS40M4	41.943	97.685	0.41