Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NYR62_RS04485 Genome accession   NZ_CP103809
Coordinates   1018817..1019263 (-) Length   148 a.a.
NCBI ID   WP_279476597.1    Uniprot ID   -
Organism   Actinobacillus genomosp. 1 strain CCUG22229     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1013817..1024263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR62_RS04460 (NYR62_04460) yacG 1014725..1014913 (-) 189 WP_279458423.1 DNA gyrase inhibitor YacG -
  NYR62_RS04465 (NYR62_04465) coaE 1014895..1015533 (-) 639 WP_279476592.1 dephospho-CoA kinase -
  NYR62_RS04470 (NYR62_04470) - 1015554..1016198 (-) 645 WP_347710247.1 prepilin peptidase -
  NYR62_RS04475 (NYR62_04475) - 1016219..1017421 (-) 1203 WP_279476595.1 type II secretion system F family protein -
  NYR62_RS04480 (NYR62_04480) pilB 1017405..1018799 (-) 1395 WP_279476596.1 GspE/PulE family protein Machinery gene
  NYR62_RS04485 (NYR62_04485) pilA 1018817..1019263 (-) 447 WP_279476597.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NYR62_RS04490 (NYR62_04490) radA 1019443..1020822 (+) 1380 WP_279458429.1 DNA repair protein RadA Machinery gene
  NYR62_RS04495 (NYR62_04495) - 1020866..1021099 (-) 234 WP_115589814.1 YgjV family protein -
  NYR62_RS04500 (NYR62_04500) folA 1021229..1021717 (-) 489 WP_279476598.1 type 3 dihydrofolate reductase -
  NYR62_RS04505 (NYR62_04505) proB 1021804..1022895 (+) 1092 WP_279458431.1 glutamate 5-kinase -
  NYR62_RS04510 (NYR62_04510) - 1022911..1023687 (+) 777 WP_279458432.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16008.39 Da        Isoelectric Point: 8.0852

>NTDB_id=725224 NYR62_RS04485 WP_279476597.1 1018817..1019263(-) (pilA) [Actinobacillus genomosp. 1 strain CCUG22229]
MQKLNLIRPLTKAFTLIELMIVIAIVAILATVAIPSYNSYTQKAALSELLSASASYKTDVEICMYNTGEAKNCSSGQNGV
SKITELKRAKYLNTITVESGTITVTGKGNLQEYGYTMTPNYNGNTISWETKCNGADLSLFPANFCAAN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=725224 NYR62_RS04485 WP_279476597.1 1018817..1019263(-) (pilA) [Actinobacillus genomosp. 1 strain CCUG22229]
ATGCAAAAACTAAATCTTATTCGACCGCTAACTAAAGCATTTACTTTAATTGAATTGATGATCGTGATTGCGATTGTCGC
CATTCTTGCTACGGTTGCGATTCCGTCATACAACAGTTATACCCAAAAAGCGGCACTTTCCGAATTGTTATCGGCGTCGG
CTTCCTATAAAACGGATGTTGAAATTTGTATGTATAACACGGGAGAGGCGAAAAATTGTAGTAGCGGTCAGAACGGCGTT
AGCAAAATTACGGAGCTTAAAAGAGCTAAATATCTAAATACGATTACGGTTGAAAGCGGCACGATTACAGTGACGGGGAA
GGGGAATCTACAGGAATACGGCTATACAATGACACCGAACTATAACGGGAATACGATTTCTTGGGAAACGAAATGTAACG
GGGCAGATTTGAGTTTATTTCCGGCAAATTTCTGTGCCGCAAATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

69.799

100

0.703
  pilA Haemophilus influenzae 86-028NP

53.741

99.324

0.534
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527