Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   NYR48_RS12270 Genome accession   NZ_CP103782
Coordinates   2529168..2530238 (-) Length   356 a.a.
NCBI ID   WP_259424265.1    Uniprot ID   -
Organism   Bacillus velezensis strain DA4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2524168..2535238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NYR48_RS12220 sipW 2524228..2524812 (-) 585 WP_015240205.1 signal peptidase I SipW -
  NYR48_RS12225 tapA 2524784..2525455 (-) 672 WP_259424260.1 amyloid fiber anchoring/assembly protein TapA -
  NYR48_RS12230 - 2525714..2526043 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  NYR48_RS12235 - 2526083..2526262 (-) 180 WP_003153093.1 YqzE family protein -
  NYR48_RS12240 comGG 2526319..2526696 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  NYR48_RS12245 comGF 2526697..2527197 (-) 501 WP_259424261.1 competence type IV pilus minor pilin ComGF -
  NYR48_RS12250 comGE 2527106..2527420 (-) 315 WP_259424262.1 competence type IV pilus minor pilin ComGE -
  NYR48_RS12255 comGD 2527404..2527841 (-) 438 WP_259424263.1 competence type IV pilus minor pilin ComGD Machinery gene
  NYR48_RS12260 comGC 2527831..2528139 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  NYR48_RS12265 comGB 2528144..2529181 (-) 1038 WP_259424264.1 competence type IV pilus assembly protein ComGB Machinery gene
  NYR48_RS12270 comGA 2529168..2530238 (-) 1071 WP_259424265.1 competence type IV pilus ATPase ComGA Machinery gene
  NYR48_RS12275 - 2530432..2531382 (-) 951 WP_259424266.1 magnesium transporter CorA family protein -
  NYR48_RS12280 - 2531528..2532829 (+) 1302 WP_021494315.1 hemolysin family protein -
  NYR48_RS12285 - 2532867..2533697 (-) 831 WP_070082115.1 STAS domain-containing protein -
  NYR48_RS12295 - 2533904..2534284 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  NYR48_RS12300 - 2534521..2534766 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40471.77 Da        Isoelectric Point: 9.3438

>NTDB_id=724861 NYR48_RS12270 WP_259424265.1 2529168..2530238(-) (comGA) [Bacillus velezensis strain DA4]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPAETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=724861 NYR48_RS12270 WP_259424265.1 2529168..2530238(-) (comGA) [Bacillus velezensis strain DA4]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGGCGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGAGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGTCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAATTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCGAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

39.08

97.753

0.382
  pilF Thermus thermophilus HB27

38.177

98.596

0.376